Recombinant Mouse Anti-VACV L1 Antibody (M7B6) (CAT#: FAMAB-0362CQ)

Figure 1 In vitro neutralization assay with anti-L1 MAbs. Anti-L1 antibodies have been tested for their ability to neutralize in vitro.

FACS-based neutralization assay featuring anti-L1 MAbs M12B9, M2E9, M7B6, 39D4, and 8C8. Anti-H3 MAbs (41 and JH4), anti-A27 MAbs (1G6 and 12G2), and anti-D8 MAbs (JE10 and LA5) were used as controls. All antibodies were used at a final concentration of 20 μg/ml. Anti-L1 MAbs 39D4 and 8C8 did not neutralize the virus in the absence or presence of complement. Anti-L1 group I MAbs (M12B9, M7B6, and M2E9) are capable of neutralizing in the absence of complement, whereas control MAbs required the presence of complement to provide neutralization.

Kaever, T., Meng, X., Matho, M. H., Schlossman, A., Li, S., Sela-Culang, I., ... & Frazier, A. (2014). Potent neutralization of vaccinia virus by divergent murine antibodies targeting a common site of vulnerability in L1 protein. Journal of virology, 88(19), 11339-11355.

Figure 2 In vitro neutralization assay with anti-L1 MAbs. Anti-L1 antibodies have been tested for their ability to neutralize in vitro.

(top) Neutralization levels for two L1 MAbs (M12B9 and M2E9) and one control MAb for A27, H3, and D8 each in the absence of complement. (Bottom) Results in the presence of complement. A 5-fold titration of antibodies was done for all antibodies, starting with a concentration of 20 g/ml (green bars). Consistent with the FACS results, the 2 anti-L1 MAbs were able to neutralize in a complementindependent manner, whereas anti-H3 MAb 41 was not able to neutralize in the absence of complement. Error bars are based on four data points per sample (averages from duplicates in two independent experiments, normalized).

Kaever, T., Meng, X., Matho, M. H., Schlossman, A., Li, S., Sela-Culang, I., ... & Frazier, A. (2014). Potent neutralization of vaccinia virus by divergent murine antibodies targeting a common site of vulnerability in L1 protein. Journal of virology, 88(19), 11339-11355.