Mouse Anti-VISTA Recombinant Antibody (clone 13F3) (CAT#: FAMAB-1565CQ)

Figure 1 VISTA-Ig–mediated suppression could overcome a moderate level of costimulation provided by CD28 but was completely reversed by a high level of costimulation, as well as partially rescued by exogenous IL-2.

(A and B) CFSE-labeled CD4+ T cells were activated by 2.5 µg/ml plate-bound ɑ-CD3 together with either VISTA-Ig or control-Ig at 1:1 and 1:2 ratios. (A) 40 ng/ml soluble mIL-2, mIL-7, mIL-15, or mIL-23 was also added as indicated to the cell culture. (B) 1 µg/ml ɑ-CD28 was immobilized together with 2.5 µg/ml ɑ-CD3 and Ig proteins at the indicated ratios. Cell proliferation was analyzed at 72 h by examining CFSE division profiles.

Wang, L., Rubinstein, R., Lines, J. L., Wasiuk, A., Ahonen, C., Guo, Y., ... & Noelle, R. J. (2011). VISTA, a novel mouse Ig superfamily ligand that negatively regulates T cell responses. Journal of Experimental Medicine, 208(3), 577-592.

Figure 2 VISTA blockade using a specific mAb enhanced CD4+ T cell response in vitro and in vivo.

Wang, L., Rubinstein, R., Lines, J. L., Wasiuk, A., Ahonen, C., Guo, Y., ... & Noelle, R. J. (2011). VISTA, a novel mouse Ig superfamily ligand that negatively regulates T cell responses. Journal of Experimental Medicine, 208(3), 577-592.