Human Anti-WNV E Recombinant Antibody (clone Fab25) (CAT#: FAMAB-0733WJ)
This product is a human monoclonal antibody that is specific for Domain III of WNV E protein. This antibody can be used in a variety of applications, such as ELISA, WB, Neut.
Specific Inquiry
ELISA
Figure 1 ELISA analysis of Fab antibodies against WNV E protein domain III.
Duan, T., Ferguson, M., Yuan, L., Xu, F., & Li, G. (2009). Human monoclonal Fab antibodies against West Nile virus and its neutralizing activity analyzed in vitro and in vivo. Journal of antivirals & antiretrovirals, 1(1), 036.
WB
Figure 2 Western blotting analysis of Fab antibodies against WNV domain III.
Duan, T., Ferguson, M., Yuan, L., Xu, F., & Li, G. (2009). Human monoclonal Fab antibodies against West Nile virus and its neutralizing activity analyzed in vitro and in vivo. Journal of antivirals & antiretrovirals, 1(1), 036.
IFA
Figure 3 IFA assay to detect WNV in Vero cells.
M: protein standards; P: positive control, human convalescent WNV serum (T-35582); N: human pooled immune globulin (without WNV antibody).
Duan, T., Ferguson, M., Yuan, L., Xu, F., & Li, G. (2009). Human monoclonal Fab antibodies against West Nile virus and its neutralizing activity analyzed in vitro and in vivo. Journal of antivirals & antiretrovirals, 1(1), 036.
Neut
Figure 4 PRNT for virus neutralization by anti-WNV Fab antibodies.
The "virus control" represents the result obtained by infection of virus only without addition of antibody. The "Negative Serum Control", "Mock Control", and "Positive Serum Control" represent the results obtained by the mixtures of virus with no anti-WNV antibody serum, supernatant of lytic XL1-blue, and anti-WNV serum, respectively. The final concentration of antibody in the virus-antibody mixture is indicated.
Duan, T., Ferguson, M., Yuan, L., Xu, F., & Li, G. (2009). Human monoclonal Fab antibodies against West Nile virus and its neutralizing activity analyzed in vitro and in vivo. Journal of antivirals & antiretrovirals, 1(1), 036.
Specifications
- Host Species
- Human
- Derivation
- Phage Display Library
- Type
- Human IgG
- Specificity
- WNV E protein
- Species Reactivity
- WNV
- Clone
- Fab25
- Applications
- ELISA, WB, Neut
- Related Disease
- WNV infection
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- West nile virus E protein; WNV E
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
Mouse Antibody Products
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Human Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-2717CQ | Recombinant Mouse Anti-WNV E Antibody (MRO-2717CQ) | IF, ELISA | Mouse antibody |
| MRO-2718CQ | Recombinant Mouse Anti-WNV E Antibody (MRO-2718CQ) | IF, ELISA | Mouse antibody |
| MRO-2719CQ | Recombinant Mouse Anti-WNV E Antibody (MRO-2719CQ) | IF, ELISA | Mouse antibody |
| MRO-2720CQ | Recombinant Mouse Anti-WNV E Antibody (VC271) | ELISA, IF, WB, IHC | Mouse antibody |
| MRO-2721CQ | Recombinant Mouse Anti-WNV E Antibody (VC272) | ELISA, IF, WB, IHC | Mouse antibody |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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