Rabbit Anti-XRCC6 Recombinant Antibody (clone R06-3F1) (CAT#: VS3-FY2019)
This marvelous product is a rabbit recombinant antibody that with high affinity in binding to human XRCC6. It is suitable for use in a variety of assays, including WB, IHC-P, IP. This R06-3F1 antibody is validated for western blot analysis of Ku70 in Hela, Ramos lysates and immunohistochemistry analysis of paraffin-embedded human tonsil.
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WB
Figure 1 Anti-XRCC6 Antibody (VS3-FY2019) in WB.
The Ku70 expression in Hela, Ramos lysates were detected using the anti-Ku70 by western blot assay.
IHC
Figure 2 Anti-XRCC6 Antibody (VS3-FY2019) in IHC.
Immunohistochemistry staining of paraffin-embedded human tonsil using the anti-Ku70 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Specifications
- Immunogen
- Synthetic peptide of human Ku70
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human XRCC6
- Species Reactivity
- Human
- Clone
- R06-3F1
- Applications
- Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 70 kDa, while the observed molecular weight stands at 70 kDa.
- Related Disease
- Lupus Erythematosus and Nijmegen Breakage Syndrome
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunoprecipitation: 1/20
Target
- Alternative Names
- X-Ray Repair Cross Complementing 6; XRCC6
- Gene ID
- 2547
- UniProt ID
- P12956
- Cellular Localization
- Chromosome, Nucleus
- Post Translation Modifications
- Phosphorylation by PRKDC may enhance helicase activity.
Phosphorylation of Ser-51 does not affect DNA repair.
ADP-ribosylated by PARP3. (P12956-XRCC6_HUMAN)
Ubiquitination at Lys123, Lys129, Lys100, Lys114, Lys182, Lys238, Lys287, Lys317, Lys351, Lys357, Lys445, Lys451, Lys461, Lys565, Lys570, Lys575, and Lys605 (NX_P12956 [NX_P12956-1])
Modification sites at PhosphoSitePlus (P12956)
Glycosylation from GlyGen (P12956) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- XP_047297260.1; XP_054181436.1; NP_001275905.1; NP_001275906.1; NP_001275907.1
- Function
- Required for double-strand break repair and V(D)J recombination.
Also has a role in chromosome translocation.
Has a role in chromosome translocation.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3700z | Mouse Anti-XRCC6 Recombinant Antibody (clone 22F9) | WB, ELISA, FC, ICC | Mouse IgG1 |
| VS3-QX1253 | Mouse Anti-XRCC6 Recombinant Antibody (clone 6H10-E9-G7) | WB, ICC | Mouse IgG2b |
| VS3-QX1254 | Mouse Anti-XRCC6 Recombinant Antibody (clone 7B1-E12-G4) | WB, ICC, IP | Mouse IgG1 |
| VS3-QX1255 | Mouse Anti-XRCC6 Recombinant Antibody (clone 1D3-E1-F9) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| VS3-FY1564 | Recombinant Rabbit Anti-XRCC6 Antibody (clone R05-1A7) | WB, IHC-Fr, IHC-P, ICC, IF | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
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