Chicken IgY Purification Kit (VS-0824-WK24)
CAT#: VS-0824-WK24
This chicken IgY purification kit purifies chicken antibodies from hen egg yolks using a unique delipidation reagent to separate protein from lipid and a precipitation reagent to selectively isolate the IgY fraction. With this kit, 80-120mg of 90% pure IgY can be easily obtained from each egg
yolk.
Specifications
- Product Size
- 1 L
- Format
- Kit
- Contents
- Delipidation reagent, 500 mL
IgY precipitation reagent, 500 mL
Egg separator, 1 separator
- Specifications
- Column type: precipitation
Purification target: IgY antibody
Capacity: 80 to 120 mg IgY per egg yolk at 85% to 95% purity by SDS-PAGE analysis
- Storage
- Store at 4°C.
- Background
- The eggs of immunized chickens are an economical and abundant source of polyclonal antibodies. A single egg may contain as much antibody as can be obtained from an average bleed of a rabbit. Immunogen-specific antibody generally is from 1-10% of the total IgY in each egg. The eggs from immunized chickens offer a nearly continual daily source of milligram quantities of specific polyclonal antibody. When using chickens, there is a higher potential of producing antibodies to mammalian antigens than when using rabbits or goats. In addition, chicken IgY does not cross-react with mammalian IgG and does not bind bacterial or mammalian Fc receptors. It has been reported, that nonspecific binding is reduced and the need to do cross-species immunoabsorptions is eliminated when using chicken antibodies
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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