Cytochrome-C Fractionation Antibody Panel (ACTB, CYCS, COX4I1)

CAT#: VS-0725-YC32

The Cytochrome-C Fractionation Antibody Panel are sets of 3 antibodies, precisely engineered for the simultaneous detection of key proteins involved in cytochrome-c release and cellular fractionation. This essential set includes antibodies against ACTB (cytoplasmic marker), CYCS (cytochrome-c), and COX4I1 (mitochondrial marker). This panel offers a streamlined approach to verify successful fractionation and monitor apoptotic events, providing confidence in your downstream analyses.

Sub Cat Component Name Clone Type Target Species Reactivity Applications  
VS-0725-YC89 Mouse Anti-COX4I1 Recombinant Antibody Mouse IgG COX4I1 Human, Mouse, Rat, Goat, Monkey FC, ICC, IF, IHC, IP, WB
VS-0725-YC184 Mouse Anti-CYCS Recombinant Antibody Mouse IgG CYCS Human, Mouse, Rat FC, ICC, IF, IP, WB
VS-0725-YC186 Mouse Anti-ACTB Recombinant Antibody Mouse IgG ACTB Human, Mouse, Rat, C. reinhardtii, Chicken, Fish, Goat, Monkey, P. pastoris, Pig, Rabbit, Rice, S. cerevisiae, Yeast, Zebrafish WB
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Specifications

  • Targets
  • ACTB, CYCS, COX4I1
  • Species Reactivity
  • Human, Mouse, Rat
  • Research Area
  • Cytochrome-C fractionation
  • Introduction
  • Cytochrome-C fractionation is a widely used biochemical technique employed to investigate the integrity of the mitochondrial outer membrane and, more importantly, to detect the release of cytochrome c from the mitochondrial intermembrane space into the cytosol. This translocation is a pivotal and often irreversible event in the initiation of the intrinsic (mitochondrial) pathway of apoptosis, a major form of programmed cell death.
  • Storage
  • Store at 4°C for up to a week. For long-term storage, store at -20°C or below.
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