Cytoplasmic / Nuclear Fraction Antibody Panel Control (COX4I1, GAPDH, HIST1H3A)
CAT#: VS-0725-YC8
The Cytoplasmic / Nuclear Fraction Antibody Panel Control are sets of 3 antibodies specifically designed for the simultaneous detection of key proteins from both cytoplasmic and nuclear compartments. Included in this essential control set are antibodies against COX4I1 (a common mitochondrial/cytoplasmic marker), GAPDH (a classic cytoplasmic marker), and HIST1H3A (a widely recognized nuclear marker). This panel offers a streamlined approach to verifying the purity of your cellular fractions, providing confidence in your downstream Western blot or immunofluorescence results.
| Sub Cat | Component Name | Clone | Type | Target | Species Reactivity | Applications | |
| VS-0725-YC89 | Mouse Anti-COX4I1 Recombinant Antibody | Mouse IgG | COX4I1 | Human, Mouse, Rat, Goat, Monkey | FC, ICC, IF, IHC, IP, WB | ||
| VS-0725-YC90 | Mouse Anti-HIST1H3A Recombinant Antibody | Mouse IgG | HIST1H3A | Human, Mouse, Rat | ICC, IF, IHC, IP, WB | ||
| VS-0725-YC91 | Mouse Anti-GAPDH Recombinant Antibody | Mouse IgG | GAPDH | Human, Mouse, Rat, Cat, Chicken, Dog, Fish, Monkey, Pig, Rabbit, X.laevis, Zebrafish | ELISA, ICC, IF, IHC, WB |
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Specifications
- Targets
- COX4I1, GAPDH, HIST1H3A
- Species Reactivity
- Human, Mouse, Rat
- Research Area
- Cytoplasmic and Nuclear Fractions
- Introduction
- Subcellular fractionation, specifically the isolation of cytoplasmic and nuclear fractions, is a fundamental biochemical technique indispensable for studying the differential localization, activity, and interactions of proteins and nucleic acids within a cell. This method leverages the distinct physical properties, primarily size and density, of cellular organelles and components to separate them through a series of controlled lysis steps and differential centrifugation. The goal is to obtain enriched samples of the cytoplasm and nucleus, free from significant contamination by other cellular compartments.
- Storage
- Store at 4°C for up to a week. For long-term storage, store at -20°C or below.
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