Cytoplasmic / Nuclear Fraction

Subcellular fractionation, specifically the isolation of cytoplasmic and nuclear fractions, is a fundamental biochemical technique indispensable for studying the differential localization, activity, and interactions of proteins and nucleic acids within a cell. This method leverages the distinct physical properties, primarily size and density, of cellular organelles and components to separate them through a series of controlled lysis steps and differential centrifugation. The goal is to obtain enriched samples of the cytoplasm and nucleus, free from significant contamination by other cellular compartments.