Deglycosylation Enzyme F (Hydrolyzes N-glycans) (CAT#: VS-0724-CJ15)
This enzyme is an amidase that hydrolyzes the asparagine(Asn, N)-linked polypeptides and high-mannose, hybrid or complex-type N-glycans. It is recommended to incubate the samples at 37 °C for 1-18 hours without denaturing conditions. The enzyme was cloned from E. meningoseptica and produced in E. coli with His-tag for easy purification.
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Specifications
- Cleavage Site
- N-linked glycans on glycoproteins
- Species Reactivity
- No species dependent
- Specificity
- Glycoproteins
- Reaction Condition
- 1 to 18-hour reaction (native condition); 15 to 60-minute reaction (denaturing condition)
- Format
- Lyophilized; Immobilized
- Original Source
- E. meningoseptica
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