Quick™ Gold-Maleimide Antibody Labeling Kit (VS-0824-FY134) (CAT#: VS-0824-FY134)

• Fluorescent Dye Name
• Gold-Maleimide

• Composition
• Diluent reagent
  Gold
  Quencher reagent
  Reaction buffer

• Procedure Steps
• Equilibrate all materials and prepared reagents to room temperature and gently stir before use.
  4.1 Dilute the biomolecule in diluent to a final volume of 40 µL (3x 2.5 µg kit) or 400 µL (1x 25 µg kit).
  4.2 Add 5 µL (3x 2.5 µg kit) or 50 µL (1x 25 µg kit) reaction buffer. Mix gently.
  4.3 Remove the cap from the Gold Mix vial, mix and pipette the sample (biomolecule in reaction buffer) directly onto the lyophilized material. Resuspend by gently aspirating and redispensing the liquid once or twice with a pipette.
  4.4 Place the cap back on the vial and place it at room temperature (20-25°C); a 30 minute incubation is recommended for proteins or antibodies and a 60 minute incubation is recommended for oligonucleotides; longer incubation times have no negative effects on the conjugate.
  4.5 After 30 minutes (or longer) of incubation, add 5 µL (3x 2.5 µg kit) or 50 µL (1x 25 µg kit) of quencher (supplied as a lyophilized product and should be reconstituted in 100 µL of water; store remaining quencher at -20°C after use).
  The conjugate is ready for use after 20 minutes. You now have 50 µL (3x 2.5 µg kit) or 500 µL (1x 25 µg kit) of 20 OD of conjugate.
  4.6 Determine the concentration of the conjugate: To determine the effective concentration of the conjugate obtained, we recommend measuring the maximum absorbance of light using a UV-Vis spectrophotometer after diluting the sample to the range appropriate for your equipment.
  Please refer to the COA of the shipped product.

• Product Precautions
• This product is intended for research purposes only and is not intended for use in human, therapeutic, or diagnostic applications.

• Kit Storage
• Upon receipt, store the kit in a dark place at a temperature of -20°C immediately. Check below for storage instructions for individual components. The kit can be stored for up to 1 year from receipt if the components have not been reconstituted. Ensure to avoid repeated freeze-thaw cycles of reagents.

• Conjugate Storage
• Storage at 4°C is advised for all conjugates. Long-term storage may benefit from the addition of a preservative. The optimal storage conditions for specific conjugates should be determined through experimentation.

• Sample Type
• Antibody sample

• Reactive Species
• The reactive species of the kit depends on the research application of the antibody.

• Fluorescent Dye Introduction
• Gold-Maleimide is a chemical substance made up of gold and maleimide covalently bonded, which exhibits good stability and reactivity and is suitable for covalent linkage in biological research. The Gold-Maleimide antibody labeling kit is a reagent kit designed for conducting experiments involving Gold-Maleimide covalent binding in the laboratory, containing necessary reagents and tools for users to perform related experimental procedures. This kit can be used in the study of interactions between biomolecules, protein structure, and function, providing researchers with convenient and effective experimental tools.

• Alter Name
• Quick™ Gold-Maleimide Antibody Labeling Kit (40 nm, 20 OD); Quick™ Gold-Maleimide Antibody Labeling Kit (10 nm, 20 OD); Quick™ Gold-Maleimide Antibody Labeling Kit (20 nm, 20 OD)

Related Products

• VS-0824-FY133
• Quick™ Gold Antibody Labeling Kit (VS-0824-FY133)