Quick™ HRP Check Kit (VS-0824-FY92)
CAT#: VS-0824-FY92
The successful conjugation of HRP to the antibody has been confirmed, and the kit used for this process has proven to be easy to use and efficient in providing quick results. This makes it a valuable tool for researchers who are working with HRP-conjugated antibodies in their studies.
Specifications
- Fluorescent Dye Name
- HRP Check
- Composition
- GAR-HRP (lyophilized)
HRP detection solution
Protein A/G Strips
Running buffer (10X)
- Procedure Steps
- After the HRP-antibody conjugation reaction, the success of the conjugation can be visually checked by running the sample on strips with the following steps:
1. Dilute the 10x running buffer with distilled water to make it 1x concentration.
2. Add 0.01% BSA to the 1x running buffer to create a final concentration.
3. Dilute the HRP-antibody conjugate in 1x running buffer with 0.01% BSA.
4. Load 40 µL of the diluted conjugate into a 96-well non-sticky plate or a suitable container.
5. Insert the strip into the well with the nitrocellulose membrane dipped into the diluted conjugate.
6. Run for 10 minutes.
7. Add 40 µL of HRP detection solution to each well.
8. Run for another 15 minutes.
9. Check the result visually.
The kit also includes a positive control consisting of lyophilized GAR-HRP, which should be run separately on a strip:
10. Reconstitute the lyophilized positive control in 50 μL dH2O to obtain a concentration of 10 μg/mL.
11. Dilute the reconstituted control to a concentration of 100 ng/mL by adding 4 µL to 396 µL of 1x running buffer + 0.01% BSA.
12. Dilute the 100 ng/mL control to 1ng/mL by adding 4 µL to 396 µL of 1x running buffer + 0.01% BSA.
13. Load 40 µL of the diluted positive control into each well.
14. Insert the strip into the well.
15. Run for 10 minutes.
16. Add 40 µL of HRP detection solution to each well.
17. Run for another 15 minutes.
18. Check the result visually.
Please refer to the COA of the shipped product.
- Product Precautions
- This product is intended for research purposes only and is not intended for use in human, therapeutic, or diagnostic applications.
- Kit Storage
- Upon arrival, please store Protein A/G Strips and the HRP detection solution at +4°C, and all other components at -20°C. The kit can be stored for up to 1 year from the date of receipt, as long as the components have not been reconstituted.
- Sample Type
- Antibody sample
- Reactive Species
- The reactive species of the kit depends on the research application of the antibody.
- Fluorescent Dye Introduction
- HRP dye, also known as horseradish peroxidase dye, is a type of fluorescent or chromogenic dye that is commonly used in biological research for the detection of proteins and other biomolecules. HRP dye is often used in enzyme-linked immunosorbent assays (ELISAs) and immunohistochemistry to visualize the presence of specific antigens or antibodies. When HRP dye is added to a sample containing the target molecule, it reacts with the enzyme to produce a colored or fluorescent signal that can be easily detected and measured. This allows researchers to quickly and accurately assess the levels and localization of specific proteins in a sample. HRP dye is known for its high sensitivity and specificity, making it a valuable tool in molecular biology and biomedical research.
- Alter Name
- Quick™ HRP Check Kit; HRP Check Kit
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Datasheet
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COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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