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Mouse Anti-GNL3 Recombinant Antibody (clone 2C11) (CAT#: VS3-XY771)

Clone 2C11 is a mouse antibody, which reacts with human GNL3 protein. This antibody is suitable for GNL3 detection by ELISA, WB, FC assays.


See more Engineered Antibody products for GNL3
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  • Gene Expression
  • Datasheet
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  • COA
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli rim.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/20770/615_A8_2_selected.jpg
Figure 2 IHC staining of human oral mucosa
Figure 2 IHC staining of human oral mucosa
(Immunohistochemical staining of human oral mucosa shows strong nucleolar positivity in squamous epithelial cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/20770/47136_A_8_1_selected.jpg
Figure 3 IF staining of human cell line A-431
Figure 3 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoli rim.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/20770/621_A8_3_blue_red_green.jpg
Figure 4 IF staining of human cell line U-251 MG
Figure 4 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoli rim.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/20770/612_A8_1_blue_red_green.jpg
Normal Tissue
Figure 5 Cerebral cortex
Figure 5 Cerebral cortex
(Neuronal cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/36742/124388_B_8_5.jpg
Figure 6 Colon
Figure 6 Colon
(Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Peripheral nerve/ganglion
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/36742/124388_A_9_3.jpg
Figure 7 Liver
Figure 7 Liver
(Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/36742/124388_A_7_4.jpg
Figure 8 Kidney
Figure 8 Kidney
(Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/36742/124388_A_9_5.jpg
Figure 9 Testis
Figure 9 Testis
(Cells in seminiferous ducts
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Leydig cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/36743/124399_A_5_6.jpg
Figure 10 Skin
Figure 10 Skin
(Fibroblasts
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Keratinocytes
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Langerhans
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Melanocytes
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/36742/124388_B_9_1.jpg
Figure 11 Lymph node
Figure 11 Lymph node
(Germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear
Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/36742/124388_A_8_8.jpg
RNA Expression
Figure 12 RNA cell line category: Low cell line specificity
Figure 12 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000163938-GNL3

Specifications

  • Immunogen
  • Recombinant fragment of human GNL3 (a.a. 1-226) expressed in E. coli.
  • Host Species
  • Mouse
  • Type
  • Mouse IgG1
  • Specificity
  • Human GNL3
  • Species Reactivity
  • Human
  • Clone
  • 2C11
  • Applications
  • Enzyme-linked Immunosorbent Assay, Western Blot, Flow Cytometry

Product Property

  • Purification
  • Affinity purified
  • Format
  • Liquid
  • Buffer
  • PBS
  • Preservative
  • 0.05% sodium azide
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • This antibody is recommended for Western Blot (1/500-1/2000), Enzyme-linked Immunosorbent Assay (1/10000) and Flow Cytometry (1/200-1/400).

Target

  • Alternative Names
  • G Protein Nucleolar 3; Nucleostemin; Guanine Nucleotide Binding Protein-Like 3 (Nucleolar); Nucleolar GTP-Binding Protein 3; Novel Nucleolar Protein 47; E2-Induced Gene 3 Protein; E2IG3

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

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Single-domain Antibody

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Recombinant Antibody

CAT Product Name Application Type
MOB-2569z Mouse Anti-GNL3 Recombinant Antibody (clone 32A10) WB, ELISA, IF Mouse IgG2a
MRO-1116-CN Rabbit Anti-GNL3 Recombinant Antibody (clone JG37-82) WB, IF, IHC, FC Rabbit IgG
VS3-XY770 Mouse Anti-GNL3 Recombinant Antibody (clone 10F10) ELISA, WB Mouse IgG1

Chicken IgY Antibody

CAT Product Name Application Type
BRD-0401MZ Chicken Anti-Nucleostemin Polyclonal IgY WB Chicken antibody
BRD-0912MZ Chicken Anti-GNL3 Polyclonal IgY WB Chicken antibody

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-1470 Hi-Affi™ Rabbit Anti-GNL3 Recombinant Antibody (clone DS1470AB) WB, ICC, FC Rabbit IgG
MOR-4581 Hi-Affi™ Rabbit Anti-GNL3 Recombinant Antibody (clone TH93DS) WB, IF, ICC, FC Rabbit IgG

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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