Mouse Anti-PAX6 Recombinant Antibody (clone 1C8)
CAT#: VS3-XY1214
Clone 1C8 is a mouse antibody, which reacts with human PAX6 protein. This antibody is suitable for PAX6 detection by ELISA, WB, FC assays.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line HEK 293
Immunofluorescent staining of human cell line HEK 293 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30775/1182_F2_2_red_green.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30775/371_A12_1_red_green.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30775/372_A12_1_red_green.jpg
RNA Expression
Figure 4 RNA cell line category: Cell line enhanced (AF22, AN3-CA, HEK93, HeLa, hTERT-RPE1, U-251 MG)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000007372-PAX6
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Specifications
- Immunogen
- Recombinant fragment of human PAX6 expressed in E. coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human PAX6
- Species Reactivity
- Human
- Clone
- 1C8
- Applications
- Enzyme-linked Immunosorbent Assay, Western Blot, Flow Cytometry
Product Property
- Format
- Liquid
- Preservative
- 0.03% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody is recommended for Western Blot (1/500-1/2000), Enzyme-linked Immunosorbent Assay (1/10000) and Flow Cytometry (1/200-1/400).
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| VS-0525-XY5207 | Anti-Mouse PAX6 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5206 | Anti-Human PAX6 Immunohistochemistry Kit | IHC |
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