Mouse Anti-RHOF Recombinant Antibody (clone 2B11D11) (CAT#: VS3-XY1371)
Clone 2B11D11 is a mouse antibody, which reacts with human RHOF protein. This antibody is suitable for RHOF detection by ELISA, WB, FC assays.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & the Golgi apparatus.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/45912/if_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & the Golgi apparatus.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/45912/if_selected.jpg
Figure 2 Colon
(Enterocytes - Mucosal lymphoid cells Staining: Medium Intensity: Moderate)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/76019/165472_A_7_3.jpg
(Enterocytes - Mucosal lymphoid cells Staining: Medium Intensity: Moderate)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/76019/165472_A_7_3.jpg
Figure 3 Kidney
(Collecting ducts Staining: Low Intensity: Moderate Quantity: <25% Distal tubules Staining: Low Intensity: Moderate Quantity: <25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/76019/165472_A_8_5.jpg
(Collecting ducts Staining: Low Intensity: Moderate Quantity: <25% Distal tubules Staining: Low Intensity: Moderate Quantity: <25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/76019/165472_A_8_5.jpg
Figure 4 Testis
(Pachytene spermatocytes Staining: Medium Intensity: Moderate Quantity:>75% Round or early spermatids Staining: Low Intensity: Weak Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/76019/165472_A_6_6.jpg
(Pachytene spermatocytes Staining: Medium Intensity: Moderate Quantity:>75% Round or early spermatids Staining: Low Intensity: Weak Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/76019/165472_A_6_6.jpg
Figure 5 Lymph node
(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/76019/165472_A_9_8.jpg
(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/76019/165472_A_9_8.jpg
Figure 6 RNA cell line category: Cell line enhanced (CAPAN-2, HDLM-2, PC-3, RPTEC TERT1)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000139725-RHOF
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000139725-RHOF
Specifications
- Immunogen
- Recombinant fragment of human RHOF (a.a. 1-84) expressed in E. coli.
- Host Species
- Mouse
- Type
- Mouse IgG2a
- Specificity
- Human RHOF
- Species Reactivity
- Human
- Clone
- 2B11D11
- Applications
- Enzyme-linked Immunosorbent Assay, Western Blot, Flow Cytometry
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody is recommended for Western Blot (1/500-1/2000), Enzyme-linked Immunosorbent Assay (1/10000) and Flow Cytometry (1/200-1/400).
Target
- Alternative Names
- Ras Homolog Family Member F, Filopodia Associated; Rho In Filopodia; Ras Homolog Gene Family, Member F (In Filopodia); Ras Homolog Family Member F (In Filopodia); Rho Family GTPase Rif; ARHF; RIF; Rho-Related GTP-Binding Protein RhoF
- Gene ID
- 54509
- UniProt ID
- Q9HBH0
Related Resources
mTOR Signaling Pathway
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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IgG Antibody Products
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Antibody Magnetic Beads
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2129z | Mouse Anti-RHOF Recombinant Antibody (clone 2B11) | WB, ELISA, FC, ICC, IF, IHC | Mouse IgG2a |
| VS3-QX971 | Mouse Anti-RHOF Recombinant Antibody (clone B6-D4) | WB, FC | Mouse IgG2a |
| VS3-XY1370 | Mouse Anti-RHOF Recombinant Antibody (clone 1D4B6) | ELISA, WB, FC | Mouse IgG2a |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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