Rabbit Anti-HSP27 (phospho S82) Antibody
CAT#: VS-0126-XL1292
Rabbit polyclonal recognizing HSP27 phosphorylated at Serine 82. This regulatory site identifies chaperone activation during cellular stress, optimized for high-performance detection in WB and IHC.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47330/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47330/945_C10_1_blue_red_green.jpg
Subcellular Location
Figure 3 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47330/953_C10_1_blue_red_green.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47331/104843_A_9_3.jpg
Normal Tissue
Figure 5 Kidney
Distal tubules
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Proximal tubules (cell body)
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47331/104843_A_7_5.jpg
Normal Tissue
Figure 6 Vagina
Squamous epithelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47331/104843_B_2_2.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (hTCEpi, MCF7)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000106211-HSPB1
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Specifications
- Host Species
- Rabbit
- Specificity
- HSPB1
- Species Reactivity
- Human
- Applications
- WB; IHC
- Conjugate
- Unconjugated
Product Property
- Clonality
- Polyclonal
- Purification
- Site-specific binders were secured via phosphopeptide affinity chromatography, while cross-reactive variants were removed through negative chromatographic depletion.
- Concentration
- 1.0 mg/mL
- Storage
- Store at -20˚C
- Shipping
- Ice Packs
Applications
- Application Notes
- Predicted MW: 27 kDa
WB: 1:500-1:1000
IHC: 1:50-1:100
Target
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Related Signaling Pathways
MAPK Signaling Pathway
Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "HSP27"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-109CT | Human Anti-HSP27 Recombinant Antibody (TAB-109CT) | WB, Inhib | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-109CT-S(P) | Human Anti-HSP27 Recombinant Antibody; scFv Fragment (TAB-109CT-S(P)) | WB, Inhib | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-109CT-F(E) | Human Anti-HSP27 Recombinant Antibody; Fab Fragment (TAB-109CT-F(E)) | WB, Inhib | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-1185MZ | Chicken Anti-Heat Shock Protein 27 (HSP27) Polyclonal IgY | WB, ICC | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-4223 | Rabbit Anti-HSP27 Recombinant Antibody (clone SI302DS) | WB, ELISA, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0043F | Mouse Anti-HSP27 Recombinant Antibody (MOB-0043F) | WB, IHC-P, IF | Mouse IgG |
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