Rabbit Anti-IKB alpha (phospho S32) Monoclonal Antibody
CAT#: VS-0126-XL163
Recognizing the critical S32 phosphorylation site of IKB alpha, this rabbit monoclonal provides targeted detection of NF-kappaB activation. It is a specialized tool primarily recommended for use in WB and IP.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3815/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human appendix
Immunohistochemical staining of human appendix shows moderate cytoplasmic positivity in lymphoid cells and glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29207/62309_A_1_2_selected.jpg
Normal Tissue
Figure 3 IHC staining of human heart muscle
Immunohistochemical staining of human heart muscle shows strong cytoplasmic positivity in myocytes.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3815/ihc_selected.jpg
Normal Tissue
Figure 4 Lymph node
Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3815/10280_A_8_8.jpg
Normal Tissue
Figure 5 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3815/10280_B_8_5.jpg
Normal Tissue
Figure 6 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3815/10280_A_8_3.jpg
Normal Tissue
Figure 7 Urinary bladder
Urothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3815/10280_A_6_5.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (RT4)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000100906-NFKBIA
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Specifications
- Host Species
- Rabbit
- Antibody Isotype
- Rabbit IgG
- Specificity
- IKB alpha (phospho S32)
- Species Reactivity
- Human
- Applications
- WB; IP
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Concentrated by applying a robust chromatography approach focused on selective isolation of the target molecules from samples.
- Concentration
- Lot specific
- Storage
- Store at -20˚C
- Shipping
- Ice Packs
Applications
- Application Notes
- WB: 1:500-1:2000
IP: 1:50
Target
- Alternative Names
- MAD3; IKBA; NF-kappa-B inhibitor alpha
- UniProt ID
- P25963
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Related Signaling Pathways
TNF Signaling Pathway
Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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| VS-0525-XY4820 | Anti-Human NFKBIA Immunohistochemistry Kit | IHC |
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| CAT | Product Name | Application | Type |
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| VS-0126-XL237 | Rabbit Anti-IKB alpha (phospho S36) Monoclonal Antibody | Phospho Antibodies for Cell Signaling Research |
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