Rabbit Anti-PFAS scFv-Fc Chimera (VS-0625-FY11)
CAT#: VS-0625-FY11
Anti-PFAS scFv-FC is a recombinant fusion protein designed for the detection and potential remediation of Per- and Polyfluoroalkyl Substances (PFAS), a group of persistent environmental contaminants. This innovative construct leverages a single-chain variable fragment (scFv) engineered to specifically bind to various PFAS compounds, allowing for their recognition and capture. The scFv is fused to the Fc domain of an immunoglobulin, typically human IgG1, which confers advantages such as increased stability, extended half-life, and potentially allows for integration into existing immunoassay formats or for facilitating separation/removal processes. This product is valuable for environmental monitoring, developing diagnostic assays for PFAS, and exploring novel approaches for PFAS detoxification or removal from contaminated matrices.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A549
Immunofluorescent staining of human cell line A549 shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/22140/1718_E1_8_cr57f3cd0b7d6bc_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Glial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/22140/51410_B_9_5.jpg
Normal Tissue
Figure 3 Colon
Glandular cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/22140/51410_A_7_3.jpg
Normal Tissue
Figure 4 Liver
Cholangiocytes Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/22140/51410_A_7_4.jpg
Normal Tissue
Figure 5 Kidney
Collecting ducts Staining: Medium Intensity: Moderate Quantity:>75% Distal tubules Staining: Medium Intensity: Moderate Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/22140/51410_A_7_5.jpg
Normal Tissue
Figure 6 Testis
Elongated or late spermatids Staining: Low Intensity: Weak Quantity: 75%-25% Leydig cells Staining: Low Intensity: Weak Quantity: 75%-25% Pachytene spermatocytes Staining: Low Intensity: Weak Quantity: 75%-25% Preleptotene spermatocytes Staining: Low Intensity: Moderate Quantity: <25% Sertoli cells Staining: Low Intensity: Weak Quantity: 75%-25% Spermatogonia cells Staining: Medium Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/22140/51410_A_6_6.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/22140/51410_A_9_8.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000178921-PFAS
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Specifications
- Immunogen
- PFOA tethered to a carrier protein (Bovine Serum Albumin - BSA) at the carboxylic acid end (PFOA-BSA conjugate).
- Host Species
- Rabbit
- Type
- Rabbit IgG, scFv-Fc
- Specificity
- It successfully binds to PFOA in both a clean matrix (2 ppb PFOA) and groundwater containing approximately 7.5 ppb of PFOA, showing a consistent and approximately proportional signal
- Applications
- ELISA
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- ELISA: 1:5000 and 1:25000
The optimal dilution concentration is determined by the experiment.
Target
- Alternative Names
- Phosphoribosylformylglycinamidine Synthase; FGAR Amidotransferase; Formylglycinamide Ribonucleotide Amidotransferase; Formylglycinamide Ribotide Amidotransferase; FGAM Synthase; EC 6.3.5.3; FGAR-AT
- Gene ID
- 5198
- UniProt ID
- O15067
- Long Name
- Phosphoribosylformylglycinamidine Synthase
- Sequence Similarities
- In the N-terminal section; belongs to the FGAMS family.
- Cellular Localization
- Cytoplasm
- Post Translation Modifications
- Ubiquitination at Lys279, Lys422, Lys451, Lys494, Lys507, Lys639, Lys646, Lys683, Lys843, Lys900, and Lys1051
Modification sites at PhosphoSitePlus
Glycosylation from GlyGen 2 sites, 1 O-linked glycan (2 sites)
- Protein Refseq
- XP_006721609.1; XP_024306572.1; XP_047292251.1; XP_047292252.1; XP_047292253.1
- Function
- Phosphoribosylformylglycinamidine synthase involved in the purines biosynthetic pathway.
Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "PFAS"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2838z | Recombinant Anti-Human PFAS Antibody | WB, IF, FuncS | IgG |
| MRO-2115-CN | Rabbit Anti-PFAS Polyclonal Antibody (MRO-2115-CN) | WB, IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2838z-F(E) | Recombinant Anti-Human PFAS Antibody Fab Fragment | FC, ELISA, FuncS | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2838z-S(P) | Recombinant Anti-Human PFAS Antibody scFv Fragment | FC, IP, FuncS | scFv |
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