Rabbit Anti-RPA2 (phospho T21) Monoclonal Antibody
CAT#: VS-0126-XL177
Specifically binding to the phosphorylated Threonine 17 epitope of Histone H1.4, this rabbit monoclonal offers precise recognition. This reagent is primarily recommended for site-specific protein screening in WB and IP.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & nuclear bodies.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26306/220_B6_1_selected.jpg
Normal Tissue
Figure 2 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16538/36325_A_8_5.jpg
Normal Tissue
Figure 3 Testis
Leydig cells Staining: High Intensity: Strong Quantity:>75% Pachytene spermatocytes Staining: High Intensity: Strong Quantity:>75% Peritubular cells Staining: High Intensity: Strong Quantity: 75%-25% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity:>75% Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: High Intensity: Strong Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16538/36325_A_6_6.jpg
Normal Tissue
Figure 4 Skin
Cells in basal layer Staining: Medium Intensity: Moderate Quantity:>75% Cells in granular layer Staining: Medium Intensity: Moderate Quantity: 75%-25% Cells in spinous layer Staining: Medium Intensity: Moderate Quantity:>75% Endothelial cells Staining: Medium Intensity: Moderate Fibrohistiocytic cells Staining: High Intensity: Strong Langerhans cells Staining: Medium Intensity: Moderate Quantity:>75% Lymphocytes Staining: High Intensity: Strong Melanocytes Staining: High Intensity: Strong Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16538/36325_B_7_1.jpg
Normal Tissue
Figure 5 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16538/36325_A_9_8.jpg
Normal Tissue
Figure 6 Colon
Endocrine cells Staining: High Intensity: Strong Endothelial cells Staining: High Intensity: Strong Quantity:>75% Enterocytes Staining: High Intensity: Strong Quantity:>75% Enterocytes - Fibroblasts Staining: High Intensity: Strong Goblet cells Staining: High Intensity: Strong Quantity:>75% Mucosal lymphoid cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16538/36325_A_9_3.jpg
Normal Tissue
Figure 7 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Hepatocytes Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16538/36325_A_8_4.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000117748-RPA2
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Specifications
- Host Species
- Rabbit
- Antibody Isotype
- Rabbit IgG
- Specificity
- RPA2 (phospho T21)
- Species Reactivity
- Human; Mouse; Rabbit
- Applications
- WB; IP
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Processed via a streamlined affinity chromatography method designed to maximize antibody yield while ensuring peak analytical clarity.
- Concentration
- Lot specific
- Storage
- Store at -20˚C
- Shipping
- Ice Packs
Applications
- Application Notes
- WB: 1:5000-1:10000
IP: 1:20
Target
- Alternative Names
- Replication protein A 32 kDa subunit; RP-A p32; REPA2; RPA32
- UniProt ID
- P15927
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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| MOB-2562z | Mouse Anti-RPA2 Recombinant Antibody (clone 30F9) | WB, ICC, IF, IHC, IP | Mouse IgG1, κ |
| CAT | Product Name | Application | Type |
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| MOB-1054CT | Recombinant Mouse anti-Human RPA2 Monoclonal antibody (3038D6) | Dot, FC, WB |
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| MRO-2371-CN | Recombinant Rabbit Anti-RPA2 (phosphorylated Thr21) Monoclonal Antibody (CBACN-657) | WB, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| VS-1024-XY454 | Mouse Anti-NHP RPA2 Recombinant Antibody (clone 9H8) | WB, IHC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
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| VS-0525-XY6184 | Anti-RPA2 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
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| VS-0525-XY6185 | Anti-Mouse RPA2 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
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| VS-0126-XL78 | Rabbit Anti-RPA32/RPA2 (phospho T21) Monoclonal Antibody | Phospho Antibodies for Cell Signaling Research |
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