Recombinant Mouse Anti-KRT7 Antibody (VS4-CJ13)
CAT#: VS4-CJ13
This mouse anti-KRT7 antibody is a product that recognizes human, dog, mouse, rat KRT7. The immunogen of this antibody is recombinant fragment, corresponding to amino acids 256aa-415aa of human KRT7 (C-terminus). The mAb has been tested for use in ELISA, Western Blot (1:500-1:5000), Immunocytochemistry (1:50-1:200).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line BJ
Immunofluorescent staining of human cell line SiHa shows localization to intermediate filaments.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7315/1891_G5_16_cr5ba0bd7759116_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line BJ
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & intermediate filaments.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/79970/1999_A12_5_selected.jpg
Subcellular Location
Figure 3 IF staining of human cell line BJ
Immunohistochemical staining of human thyroid gland shows strong cytoplasmic and membranous positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/79970/170875_B_3_5_selected.jpg
Normal Tissue
Figure 4 Liver
Cholangiocytes
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7272/18504_A_8_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules
Staining:
High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7272/18504_A_8_5.jpg
Normal Tissue
Figure 6 Breast
Glandular cells
Staining:
High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/28/299_B_2_4.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (A549, BEWO, HBEC3-KT, hTERT-HME1, RT4, SiHa, SK-BR-3)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000135480-KRT7
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Specifications
- Immunogen
- Recombinant fragment, corresponding to amino acids 256aa-415aa of human KRT7 (C-terminus).
- Host Species
- Mouse
- Type
- Mouse IgG2b
- Specificity
- Human, Dog, Mouse, Rat KRT7
- Species Reactivity
- Human, Dog, Mouse, Rat
- Applications
- ELISA, WB, ICC
- Conjugate
- Unconjugated
Product Property
- Purification
- Affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 50% Glycerol, 0.1M Tris-Glycine (150 mM NaCl), pH 7.4.
- Preservative
- 0.2% Sodium Azide
- Storage
- Store at -20°C or -80°C upon receipt. Avoid repeated freeze.
Applications
- Application Notes
- This antibody has been tested for use in ELISA, Western Blot (1:500-1:5000), Immunocytochemistry (1:50-1:200).
Target
- Alternative Names
- K7; CK7; SCL; K2C7
- Gene ID
- 3855
- UniProt ID
- P08729
- Long Name
- Keratin 7
- Sequence Similarities
- Belongs to the intermediate filament family.
- Cellular Localization
- Cytoplasm, Intermediate filament, Keratin
- Post Translation Modifications
- Arg-20 is dimethylated, probably to asymmetric dimethylarginine.
- Protein Refseq
- NP_005547.3
- Function
- Blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
See other products for "KRT7"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1992 | Hi-Affi™ Recombinant Rabbit Anti-KRT7 Monoclonal Antibody (DS1992AB) | IHC-P | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0469F | Mouse Anti-KRT7 Recombinant Antibody (ZG-0469F) | WB, IF | Mouse IgG |
| ZG-0470F | Mouse Anti-KRT7 Recombinant Antibody (ZG-0470F) | WB, IF | Mouse IgG |
| VS3-QX663 | Mouse Anti-KRT7 Recombinant Antibody (clone 4G9-D8-H6) | WB, ICC | Mouse IgG2b |
| VS3-QX664 | Mouse Anti-KRT7 Recombinant Antibody (clone 6E10-D1-A5) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| VS3-XY3412 | Rabbit Anti-KRT7 Recombinant Antibody (clone R09-7D1) | WB, IHC-P, ICC, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1383 | AbPlus™ Anti-KRT7 Magnetic Beads (VS-0724-YC1383) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3884 | Anti-KRT7 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3885 | Anti-Mouse KRT7 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3887 | Anti-Rat KRT7 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3888 | Anti-Canine KRT7 Immunohistochemistry Kit | IHC |
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