What is the specificity of the Recombinant Human Anti-LY6G6D Antibody (clone 8I8)?

The Recombinant Human Anti-LY6G6D Antibody (clone 8I8) targets the LY6G6D protein, which is linked to the cell surface via glycosylphosphatidylinositol (GPI). It has little to no cross-reactivity with other LY6 family proteins, according to comprehensive testing.

How should I prepare my samples for Flow Cytometry using this antibody?

For Flow Cytometry, create a single-cell suspension and stain cells with the necessary antibody concentration (often 0.1-5 µg per million cells, depending on the primary staining process). Incubate the cells with the antibody for 30 minutes at 4°C, then wash thoroughly to eliminate any unbound antibody.

Recombinant Human Anti-LY6G6D Antibody (clone 8I8)

Specifications

Immunogen
Recombinant Human LY6G6D protein

Host Species
Human

Type
Human IgG1

Specificity
Human, Monkey LY6G6D

Species Reactivity
Human, Monkey

Clone
8I8

Applications
ELISA, FC

Conjugate
Unconjugated

Product Property

Purification
Affinity chromatography

Purity
>95% as determined by SDS-PAGE

Format
Liquid

Buffer
50% Glycerol, 0.01M PBS, pH 7.4.

Preservative
0.03% Proclin 300

Storage
Store at -20°C or -80°C upon receipt. Avoid repeated freeze.

Target

Alternative Names
Lymphocyte antigen 6 complex locus protein G6d; Protein Ly6-D; Megakaryocyte-enhanced gene transcript 1 protein; LY6G6D; C6orf23; G6D; MEGT1; NG25; NG32

Gene ID
58530

UniProt ID
O95868

Long Name
Lymphocyte antigen 6 family member G6D

Cellular Localization
Cell membrane, Cell projection, Membrane

Post Translation Modifications
O-glycosylated.

Protein Refseq
NP_067069.2

Recommended Products

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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Non-Human Primate (NHP) Antibody Products

CAT	Product Name	Application	Type
VS-1024-XY311	Mouse Anti-NHP LY6G6D Recombinant Antibody (clone 3D6E10)	WB, IF, FC, ELISA	Mouse IgG1

Customer Reviews and Q&As

Customer Review Q&As

Excellent for FC

I have been using this antibody for my flow cytometry assays, and the results have been phenomenal. I get a clear and distinct signal with minimal background noise. The specificity is impressive, making it easier to identify the cell populations of interest. Highly recommended for anyone working with LY6G6D markers.

Great for Western Blots

Excellent antibody for western blot applications. The antibody produced distinct bands and consistent results across numerous samples. The only reason I'm giving it four stars is because of the price, which I believe is little higher than other antibodies, but the quality makes it worthwhile.

Suitable for FC

Customer service was excellent, and the technical support team was incredibly helpful when I had questions about optimizing my protocol. The Recombinant Human Anti-LY6G6D Antibody (clone 8I8) performed excellently in my flow cytometry experiments, providing clear and sharp results. This has become my go-to antibody for LY6G6D detection