Recombinant Mouse Anti-MYOD1 Antibody (clone 3B4C3)
CAT#: VS3-FY996
This product is a recombinant mouse antibody that recognizes MYOD1. This antibody has been reported for use in Enzyme-linked Immunosorbent Assay, Immunocytochemistry, Flow Cytometry. The clone 3B4C3 is specific for MYOD1.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line LHCN-M2
Immunofluorescent staining of human cell line LHCN-M2 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55436/1781_C6_34_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line RH-30
Immunofluorescent staining of human cell line RH-30 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/78647/1807_A12_34_selected.jpg
RNA Expression
Figure 3 RNA cell line category: Cell line enriched (RH-30)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000129152-MYOD1
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Specifications
- Host Species
- Mouse
- Type
- Mouse IgG1
- Clone
- 3B4C3
- Applications
- Enzyme-linked Immunosorbent Assay, Immunocytochemistry, Flow Cytometry
- Conjugate
- Unconjugated
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Enzyme-linked Immunosorbent Assay, Immunocytochemistry, Flow Cytometry.
Target
- Alternative Names
- Myogenic Differentiation 1; Myogenic Factor 3; Class C Basic Helix-Loop-Helix Protein 1; Myoblast Determination Protein 1; BHLHc1; Myf-3; MYF3; MYOD; PUM
- Gene ID
- 4654
- UniProt ID
- P15172
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Phosphorylated by CDK9. This phosphorylation promotes its function in muscle differentiation.
Acetylated by a complex containing EP300 and PCAF. The acetylation is essential to activate target genes. Conversely, its deacetylation by SIRT1 inhibits its function (By similarity).
Ubiquitinated on the N-terminus; which is required for proteasomal degradation.
Methylation at Lys-104 by EHMT2/G9a inhibits myogenic activity.
- Protein Refseq
- NP_002469.2
- Function
- Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation. Together with MYF5 and MYOG, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins (By similarity).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "MYOD1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2296z | Mouse Anti-MYOD1 Recombinant Antibody (clone 24G3) | FC, IF, IHC | Mouse IgG1, κ |
| MOB-0329MZ | Recombinant Mouse Anti-Human Myogenic Differentiation 1 Antibody (clone 6.9B) | IHC | Mouse antibody |
| MRO-1054-CN | Recombinant Rabbit Anti-MYOD1 Monoclonal Antibody (JM10-72) | WB, IP, FC | Rabbit IgG |
| ZG-0730F | Mouse Anti-MYOD1 Recombinant Antibody (clone 5.2F) | ELISA, WB, IP, IF, IHC | Mouse IgG2a |
| VS11-QX115 | Mouse Anti-MYOD1 Recombinant Antibody (clone 6F4-2D7) | IHC, WB, ELISA | Mouse IgG2a, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2352 | Hi-Affi™ Recombinant Rabbit Anti-MYOD1 Monoclonal Antibody (DS2352AB) | WB, IP, FC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-QX798 | Mouse Anti-MYOD1 Recombinant Antibody (clone 598CT21.2.4) | WB | Mouse IgM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY196 | AbPlus™ Anti-MYOD1 Magnetic Beads (5.2F) | IP, Protein Purification | |
| VS-0724-YC669 | AbPlus™ Anti-MYOD1 Magnetic Beads (VS-0724-YC669) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4657 | Anti-MYOD1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4658 | Anti-Mouse MYOD1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4659 | Anti-Porcine MYOD1 Immunohistochemistry Kit | IHC |
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