Recombinant Rabbit Anti-NEFH Capture Antibody (clone R92-8B-9)
CAT#: VS3-WK1882
This antibody is a rabbit recombinant antibody against human NEFH. It was developed by immunization of rabbit with recombinant human NEFH protein and then was produced as a recombinant antibody. The isotype of this product is rabbit IgG. The sandwich ELISA was established using matched antibody pair R92-8B-9 and R11-1D-9. The clone R92-8B-9 can be used as a capture antibody in ELISA analysis to identify human NEFH.
Gene Expression
Normal Tissue
Figure 1 High expression in cerebral cortex
Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7786/20899_B_7_5.jpg
Normal Tissue
Figure 2 Colon
Glandular cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7786/20899_A_7_3.jpg
Normal Tissue
Figure 3 Liver
Hepatocytes Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7786/20899_A_9_4.jpg
Normal Tissue
Figure 4 Prostate
Glandular cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7786/20899_A_1_5.jpg
RNA Expression
Figure 5 RNA cell line category: Cell line enriched (SiHa)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000100285-NEFH
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Specifications
- Immunogen
- Recombinant human NEFH protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human NEFH
- Species Reactivity
- Human
- Clone
- R92-8B-9
- Applications
- ELISA
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Aggregation
- < 5%
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 0.01M PBS, pH 7.2
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Target
- Alternative Names
- Neurofilament Heavy; Neurofilament, Heavy Polypeptide 200kDa; Neurofilament Heavy Polypeptide; Neurofilament Triplet H Protein; 200 KDa Neurofilament Protein; NF-H; NFH; KIAA0845; CMT2CC
- Gene ID
- 4744
- UniProt ID
- P12036
- Long Name
- Neurofilament Heavy
- Sequence Similarities
- Belongs to the intermediate filament family.
- Cellular Localization
- Cell projection, Cytoplasm, Cytoskeleton, Intermediate filament
- Post Translation Modifications
- There are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.
Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincidentally with a change in the neurofilament function.
- Protein Refseq
- NP_066554.2
- Function
- Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. NEFH has an important function in mature axons that is not subserved by the two smaller NF proteins. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks (By similarity).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Detection antibody
Isotype Control
- CAT
- Product Name
Secondary Antibody
See other products for "NEFH"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1632z | Mouse Anti-NEFH Recombinant Antibody (clone 27C8) | WB, ELISA, FC, ICC, IF, IHC | Mouse IgG1 |
| MRO-1078-CN | Recombinant Rabbit Anti-NEFH Monoclonal Antibody (JF99-07) | WB, IHC | Rabbit IgG |
| MRO-2067-CN | Rabbit Anti-NEFH Polyclonal Antibody (MRO-2067-CN) | WB, IF, IHC | Rabbit IgG |
| VS3-QX805 | Mouse Anti-NEFH Recombinant Antibody (clone 8H10) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| VS-0223-WK51 | Rabbit Anti-NEFH Recombinant Antibody (VS-0223-WK51) | ICC, IHC, IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0719MZ | Chicken Anti-NEFH Polyclonal IgY | ICC, IF, IHC, WB | Chicken antibody |
| BRD-1121MZ | Chicken Anti-Neurofilament H (NF-H) Polyclonal IgY | WB, IF | Chicken antibody |
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