Recombinant Anti-PTCH1 Vesicular Antibody, EV Displayed (VS-0425-YC391)
CAT#: VS-0425-YC391
The Recombinant Anti-PTCH1 Vesicular Antibody, EV Displayed (VS-0425-YC391) is an antibody-displaying extracellular vesicle (Ab-EV). The product combines the benefits of both extracellular vesicle (EV) and antibody (Ab) which can guide the decorated EVs to PTCH1-expressed cells or tissues. The PTCH1 is a component of the hedgehog signaling pathway, crucial for development and tumorigenesis, with mutations linked to basal cell nevus syndrome.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/75072/1664_C6_2_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human duodenum
Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13717/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Glial cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous
Neuronal cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Neuropil
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13717/30252_B_7_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13717/30242_A_7_3.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Hepatocytes
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13717/30242_A_8_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13717/30242_A_7_5.jpg
Normal Tissue
Figure 7 Testis
Cells in seminiferous ducts
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13717/30242_A_6_6.jpg
Normal Tissue
Figure 8 Lymph node
Germinal center cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13717/30242_A_9_8.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enhanced (NTERA-2, WM-115)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000185920-PTCH1/subcellular
❮
❯
Recombinant Antibody
- Application
- ELISA, FC, SPR, Cell-uptake
- Product Type
- Ab-Fc-EVs
- Antibody Quantification (Ab/EV)
- ~100 Ab/EV
- Target
- PTCH1
- Host Animal
- Human
- Antibody Isotype
- IgG1
- Species Reactivity
- Human
- Expression Cell
- Mammalian cell
Engineered EVs
- EV-sorting domain
- CD63
- Fc-binding domain
- z domain
- EV Size
- 30~150 nm
- Producing Cell
- HEK293F
- Isolation Method
- Gradient centrifugation
- Purification
- qEV size exclusion chromatography
- Binding Affinity
- Kd = 0.85 µg/mL
- Concentration
- 1 x 10¹⁰
- Size
- 1 mL
- Buffer
- PBS
- Storage
- Store at -80°C for 12 months
Target
- Full Name
- Patched 1
- Molecular Function
- Receptor
- Cellular Localization
- Golgi apparatus, Primary cilium
- Introduction
- This gene encodes a member of the patched family of proteins and a component of the hedgehog signaling pathway. Hedgehog signaling is important in embryonic development and tumorigenesis. The encoded protein is the receptor for the secreted hedgehog ligands, which include sonic hedgehog, indian hedgehog and desert hedgehog. Following binding by one of the hedgehog ligands, the encoded protein is trafficked away from the primary cilium, relieving inhibition of the G-protein-coupled receptor smoothened, which results in activation of downstream signaling. Mutations of this gene have been associated with basal cell nevus syndrome and holoprosencephaly.
- Alternative Names
- BCNS, NBCCS, PTCH
- Gene ID
- 5727
- UniProt ID
- Q13635
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Datasheet
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COA
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See other products for "PTCH1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-4063z | Mouse Anti-PTCH1 Recombinant Antibody (clone 27G5) | WB, ICC, IF, IHC | Mouse IgG2b |
| MOB-1553CT | Recombinant Mouse anti-Human PTCH1 Monoclonal antibody (EML647) | WB | |
| PABS-0285 | Camelid Anti-PTCH1 Recombinant Antibody (clone NB75) | SPR, Isothermal titration microcalorimertry (ITC), ELISA | Camelid VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NBD-0076-YJ-VHH | Camelid Anti-PTCH1 Recombinant Single Domain Antibody (NBD-0076-YJ-VHH) | ELISA | Camelid VHH |
| NBD-0077-YJ-VHH | Camelid Anti-PTCH1 Recombinant Single Domain Antibody (NBD-0077-YJ-VHH) | ELISA | Camelid VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5817 | Anti-PTCH1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5818 | Anti-Mouse PTCH1 Immunohistochemistry Kit | IHC |
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