Recombinant Mouse Anti-RPA1 Antibody (VS4-CJ104) (CAT#: VS4-CJ104)

• Immunogen
• Purified recombinant human RPA70 protein fragments expressed in E. coli.

• Host Species
• Mouse

• Type
• Mouse IgG2a

• Specificity
• Human, Monkey, Mouse, Rat RPA1

• Species Reactivity
• Human, Monkey, Mouse, Rat

• Applications
• ELISA, WB, ICC, IP

• Conjugate
• Unconjugated

• Purification
• Affinity purified

• Purity
• >95% as determined by SDS-PAGE

• Format
• Liquid

• Buffer
• 50% Glycerol, PBS, pH 7.4.

• Preservative
• 0.02% Sodium Azide

• Storage
• Store at -20°C or -80°C upon receipt. Avoid repeated freeze.

• Application Notes
• This antibody has been tested for use in ELISA, Western Blot (1:500-1:5000), Immunocytochemistry (1:50-1:200Immunoprecipitation (1:200-1:2000).

• Alternative Names
• HSSB; RF-A; RP-A; REPA1; RPA70; MST075; PFBMFT6

• Gene ID
• 6117

• UniProt ID
• P27694

• Long Name
• Replication protein A1

• Sequence Similarities
• Belongs to the replication factor A protein 1 family.

• Cellular Localization
• Nucleus

• Post Translation Modifications
• DNA damage-induced 'Lys-63'-linked polyubiquitination by PRPF19 mediates ATRIP recruitment to the RPA complex at sites of DNA damage and activation of ATR (PubMed:24332808).
  Ubiquitinated by RFWD3 at stalled replication forks in response to DNA damage: ubiquitination by RFWD3 does not lead to degradation by the proteasome and promotes removal of the RPA complex from stalled replication forks, promoting homologous recombination (PubMed:26474068).
  Sumoylated on lysine residues Lys-449 and Lys-577, with Lys-449 being the major site. Sumoylation promotes recruitment of RAD51 to the DNA damage foci to initiate DNA repair through homologous recombination. Desumoylated by SENP6.

• Protein Refseq
• NP_002936.1

• Function
• As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism (PubMed:27723720, PubMed:27723717).
  Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage (PubMed:9430682).
  In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response (PubMed:24332808).
  It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage (PubMed:17765923).
  Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair (PubMed:7697716).
  Also plays a role in base excision repair (BER) probably through interaction with UNG (PubMed:9765279).
  Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. Plays a role in telomere maintenance (PubMed:17959650, PubMed:34767620).
  As part of the alternative replication protein A complex, aRPA, binds single-stranded DNA and probably plays a role in DNA repair. Compared to the RPA2-containing, canonical RPA complex, may not support chromosomal DNA replication and cell cycle progression through S-phase. The aRPA may not promote efficient priming by DNA polymerase alpha but could support DNA synthesis by polymerase delta in presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange (PubMed:19996105).

Secondary Antibody

• 2RD-A1464-YJ
• Goat Anti-Mouse IgG2a Secondary antibody

• 2RD-A1564-YJ
• Rabbit Anti-Mouse IgG2a Secondary antibody (Clone RM219)

Recommended Dilution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS

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