Recombinant Mouse Anti-SLC2A1 Antibody (clone 1G5-8F2-7D10)
CAT#: VS3-FY1346
This product is a recombinant mouse antibody that recognizes SLC2A1. This antibody has been reported for use in Immunohistochemistry-Paraffin. The clone 1G5-8F2-7D10 is specific for human SLC2A1. The antigen is synthetic peptide derived from human glut-1.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/31345/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line Hep G2
Immunofluorescent staining of human cell line Hep G2 shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/58494/if_selected.jpg
Normal Tissue
Figure 3 High expression in placenta
Trophoblastic cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/31345/116018_A_2_7.jpg
Normal Tissue
Figure 4 Cerebral cortex
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/58494/133001_B_9_5.jpg
RNA Expression
Figure 5 RNA cell line category: Cell line enhanced (BEWO, CACO-2, Hep G2)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000117394-SLC2A1
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IHC-P
Figure 1. Recombinant Mouse Anti-SLC2A1 Antibody (clone 1G5-8F2-7D10) in IHC-P.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using an antibody to the glucose transporter GLUT1. High pressure and high temperature sodium citrate pH 6.0 for antigen retrieval.
IHC-P
Figure 2. Recombinant Mouse Anti-SLC2A1 Antibody (clone 1G5-8F2-7D10) in IHC-P.
Immunohistochemical analysis of paraffin-embedded human placenta using an antibody to the glucose transporter GLUT1. High pressure and high temperature sodium citrate pH 6.0 for antigen retrieval.
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Specifications
- Immunogen
- Synthetic peptide derived from human GLUT-1.
- Host Species
- Mouse
- Type
- Mouse IgG3
- Specificity
- Human SLC2A1
- Species Reactivity
- Human
- Clone
- 1G5-8F2-7D10
- Applications
- Immunohistochemistry-Paraffin
- Conjugate
- Unconjugated
Product Property
- Purification
- Affinity Chromatography
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- PBS, pH 7.3, 50% glycerol, 0.5% BSA
- Preservative
- 0.02% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Immunohistochemistry-Paraffin: 1/100-1/500
Target
- Alternative Names
- Solute Carrier Family 2 Member 1; Choreoathetosis/Spasticity, Episodic (Paroxysmal Choreoathetosis/Spasticity); Solute Carrier Family 2 (Facilitated Glucose Transporter), Member 1; Human T-Cell Leukemia Virus (I And II) Receptor; Glucose Transporter Type 1, Erythrocyte/Brain; HepG2 Glucose Transporter; GLUT-1; GLUT1; Solute Carrier Family 2, Facilitated Glucose Transporter Member 1; Receptor For HTLV-1 And HTLV-2; GLUT1DS
- Gene ID
- 6513
- UniProt ID
- P11166
- Sequence Similarities
- Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.
- Cellular Localization
- Cell membrane, Membrane
- Post Translation Modifications
- Phosphorylation at Ser-226 by PKC promotes glucose uptake by increasing cell membrane localization.
- Protein Refseq
- NP_006507.2
- Function
- Facilitative glucose transporter, which is responsible for constitutive or basal glucose uptake (PubMed:18245775, PubMed:19449892, PubMed:25982116, PubMed:27078104, PubMed:10227690).
Has a very broad substrate specificity; can transport a wide range of aldoses including both pentoses and hexoses (PubMed:18245775, PubMed:19449892).
Most important energy carrier of the brain: present at the blood-brain barrier and assures the energy-independent, facilitative transport of glucose into the brain (PubMed:10227690).
In association with BSG and NXNL1, promotes retinal cone survival by increasing glucose uptake into photoreceptors (By similarity).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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