Anti-TM4SF1 (clone v1.10)-mc-LP2 ADC
CAT#: ADC-074LZY
This ADC product is composed of an anti-TM4SF1 antibody (clone v110) conjugated via mc linker to LP2 (v110-mc-LP2). It has demonstrated a response in TM4SF1-expressing tumor (NSCLC and pancreas, prostateand colon cancers) treatment by a MOA (Mechanism of Action) of LP2 inhibiting tubulin polymerization andultimately inducing G2-M cell-cycle arrest and cell death .
Gene Expression
Normal Tissue
Figure 1 IHC staining of human fallopian tube
Immunohistochemical staining of human fallopian tube shows moderate membranous positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2823/8289_A_7_7_selected.jpg
Normal Tissue
Figure 2 IHC staining of human fallopian tube
Immunohistochemical staining of human fallopian tube shows strong cytoplasmic positivity with a granular pattern in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2760/7039_A_7_7_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2823/8289_B_7_5.jpg
Normal Tissue
Figure 4 Colon
Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2823/8289_A_9_3.jpg
RNA Expression
Figure 5 RNA cell line category: Cell line enhanced (CAPAN-2, hTERT-RPE1, HUVEC TERT2, LHCN-M2, OE19)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000169908-TM4SF1
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Specifications
- Antibody Overview
- Humanized Anti-Human TM4SF1 mAb, v1.10
- Clone
- v110
- Antibody Conjugation
- ADCs were prepared by partial reduction of the antibodies with tris(2-carboxyethyl)phosphine followed by
coupling to maleimidocapronic-auristatin (mc3377) (33). Excess of N-ethylmaleimide and L-Cys were added in
sequence to cap the unreacted thi
- Linker
- mc(maleimidocaproyl)
- Linker Class/Description
- Class: Noncleavable linker
Description: Noncleavable linker is considered noncleavable-meaning linker cleavage, and payload release does not depend on the differential properties between the plasma and some cytoplasmic compartments. Instead, the release of the cytotoxic drug is postulated to occur after internalization of the ADC via antigen-mediated endocytosis and delivery to lysosomal compartment, where the antibody is degraded to the level of amino acids through intracellular proteolytic degradation
- Drug
- LP2 (chemical name mc-3377)
- Drug Class/Description
- Class: Auristatin
Description: Auristatins are water-soluble dolastatin analogs of dolastatin 10. Dolastatin 10 belongs to dolastatin family and it can powerfully bind to tubulin, thus inhibiting polymerization mediated through the binding to the vinca alkaloid binding domain, and causes cell to accumulate in metaphase arrest.
Target
- Introduction
- The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface antigen and is highly expressed in different carcinomas. [provided by RefSeq, Jul 2008]
- Alternative Names
- TM4SF1; transmembrane 4 L six family member 1; L6; H-L6; M3S1; TAAL6; transmembrane 4 L6 family member 1; tumor-associated antigen L6; transmembrane 4 superfamily member 1; membrane component chromosome 3 surface marker 1; membrane component, chromosome 3, surface marker 1;
- Gene ID
- 4071
- UniProt ID
- P30408
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Effective Against TM4SF1 Targets
We used this ADC to target TM4SF1 in non-small cell lung cancer cell lines. The antibody v1.10 recognized the surface antigen efficiently. The LP2 payload successfully induced G2-M cell-cycle arrest in our proliferation assays, providing clear and reproducible results.
Robust Noncleavable Linker Design
The maleimidocaproyl linker used here is truly noncleavable as described. Our degradation studies showed that the drug release is dependent on the catabolism of the antibody backbone, which was exactly the mechanism we wished to investigate for this target.
Q&As
-
What is the mechanism of action of the LP2 payload?
A: The LP2 payload (chemical name mc-3377) is an auristatin derivative. It functions by inhibiting tubulin polymerization, which ultimately induces G2-M cell-cycle arrest and leads to cell death in the targeted cells.
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Is the mc linker in this product cleavable?
A: No, the mc (maleimidocaproyl) linker is a noncleavable linker. The release of the drug is postulated to occur after the ADC is internalized via antigen-mediated endocytosis and the antibody is degraded to the level of amino acids in the lysosome.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
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Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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See other products for "TM4SF1"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3551z | Mouse Anti-TM4SF1 Recombinant Antibody (clone 41H4) | FC, ICC, IHC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-692CT | Mouse Anti-TM4SF1 Recombinant Antibody (TAB-692CT) | ELISA, FC, IHC | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-692CT-S(P) | Mouse Anti-TM4SF1 Recombinant Antibody; scFv Fragment (TAB-692CT-S(P)) | ELISA, FC, IHC | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-692CT-F(E) | Mouse Anti-TM4SF1 Recombinant Antibody; Fab Fragment (TAB-692CT-F(E)) | ELISA, FC, IHC | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0723-WK10 | Human Anti-TM4SF1 Recombinant Antibody (VS-0723-WK10) | FC | Human IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC102 | Recombinant Anti-TM4SF1 Vesicular Antibody, EV Displayed (VS-0425-YC102) | ELISA, FC, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY7317 | Anti-TM4SF1 Immunohistochemistry Kit | IHC |
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