10-FnIII scaffold protein anti-Human Abelson (Abl) kinase SH2 domain (CAT#: SFN-L001)
The tenth fibronectin type III domain (10Fn3) that bound Abelson (Abl) kinase SH2 domain was isolated from the library using Phage display technology. The fibronectin type III domain (FN3) is a small autonomous folding unit which occurs in many animal proteins involving in ligand binding. The β-sandwich structure of FN3 closely resembles that of immunoglobulin domains. After affinity maturation, 10Fn3 variants were identified having affinities (KD=7 nM) for Abelson (Abl) kinase SH2 domain as determined by biosensor technology. It's potential to be used in diagnostic, research and therapeutic applications.
Specific Inquiry
Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & nuclear bodies.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/27280/259_B8_2_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & nuclear bodies.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/27280/259_B8_2_selected.jpg
Figure 2 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/28409/497_A9_2_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/28409/497_A9_2_selected.jpg
Figure 3 IHC staining of human heart muscle
(Immunohistochemical staining of human heart muscle shows strong cytoplasmic positivity in myocytes.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/27251/ihc_selected.jpg
(Immunohistochemical staining of human heart muscle shows strong cytoplasmic positivity in myocytes.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/27251/ihc_selected.jpg
Figure 4 IHC staining of human prostate
(Immunohistochemical staining of human prostate shows cytoplasmic and membranous positivity in glandular cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/27280/ihc_selected.jpg
(Immunohistochemical staining of human prostate shows cytoplasmic and membranous positivity in glandular cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/27280/ihc_selected.jpg
Figure 5 IHC staining of human adrenal gland
(Immunohistochemical staining of human adrenal gland shows strong nuclear positivity in cortical cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/28409/ihc_selected.jpg
(Immunohistochemical staining of human adrenal gland shows strong nuclear positivity in cortical cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/28409/ihc_selected.jpg
Figure 6 IHC staining of human duodenum
(Immunohistochemical staining of human duodenum shows moderate cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2686/ihc_selected.jpg
(Immunohistochemical staining of human duodenum shows moderate cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2686/ihc_selected.jpg
Figure 7 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & nuclear bodies.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/27280/258_B8_1_red_green.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & nuclear bodies.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/27280/258_B8_1_red_green.jpg
Figure 8 Cerebral cortex
(Neuropil
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/28409/61378_B_8_5.jpg
(Neuropil
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/28409/61378_B_8_5.jpg
Figure 9 Adrenal gland
(Glandular cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/28409/61378_B_5_5.jpg
(Glandular cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/28409/61378_B_5_5.jpg
Figure 10 Colon
(Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Peripheral nerve/ganglion
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/28409/61494_A_9_3.jpg
(Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Peripheral nerve/ganglion
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/28409/61494_A_9_3.jpg
Figure 11 Liver
(Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/28409/61494_A_9_4.jpg
(Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/28409/61494_A_9_4.jpg
Figure 12 Kidney
(Cells in glomeruli
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/28409/61494_A_9_5.jpg
(Cells in glomeruli
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/28409/61494_A_9_5.jpg
Figure 13 Testis
(Spermatogonia cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/28409/61494_A_6_6.jpg
(Spermatogonia cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/28409/61494_A_6_6.jpg
Figure 14 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000097007-ABL1
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000097007-ABL1
Specifications
- Scaffold Name
- 10-FnIII
- Origin
- Fibronectin
- Core Structure
- β-sandwich of 7 β-strands
- Variable Regions
- 2 Loops
- Target
- Abelson (Abl) kinase SH2 domain
- Species Reactivity
- Human
- Expression Host
- BL21 (DE3) cells
- Affinity Constant
- 7 nM
- Applications
- ELISA; immunohistochemistry; Microscopy; Flow Cytometer; Western Blotting; Functional Study
Target
- Alternative Names
- Fibronectin; 10-FnIII; ABL; JTK7; p150; c-ABL; v-abl; c-ABL1; bcr/abl
Related Resources
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For Research Use Only. Not For Clinical Use.
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