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    10-FnIII scaffold protein anti-Human Abelson (Abl) kinase SH2 domain (CAT#: SFN-L001)

    The tenth fibronectin type III domain (10Fn3) that bound Abelson (Abl) kinase SH2 domain was isolated from the library using Phage display technology. The fibronectin type III domain (FN3) is a small autonomous folding unit which occurs in many animal proteins involving in ligand binding. The β-sandwich structure of FN3 closely resembles that of immunoglobulin domains. After affinity maturation, 10Fn3 variants were identified having affinities (KD=7 nM) for Abelson (Abl) kinase SH2 domain as determined by biosensor technology. It's potential to be used in diagnostic, research and therapeutic applications.

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    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location and Protein Expression
    Figure 1 IF staining of human cell line A-431
    Figure 1 IF staining of human cell line A-431
    (Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & nuclear bodies.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/27280/259_B8_2_selected.jpg
    Figure 2 IF staining of human cell line U-2 OS
    Figure 2 IF staining of human cell line U-2 OS
    (Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/28409/497_A9_2_selected.jpg
    Figure 3 IHC staining of human heart muscle
    Figure 3 IHC staining of human heart muscle
    (Immunohistochemical staining of human heart muscle shows strong cytoplasmic positivity in myocytes.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/27251/ihc_selected.jpg
    Figure 4 IHC staining of human prostate
    Figure 4 IHC staining of human prostate
    (Immunohistochemical staining of human prostate shows cytoplasmic and membranous positivity in glandular cells.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/27280/ihc_selected.jpg
    Figure 5 IHC staining of human adrenal gland
    Figure 5 IHC staining of human adrenal gland
    (Immunohistochemical staining of human adrenal gland shows strong nuclear positivity in cortical cells.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/28409/ihc_selected.jpg
    Figure 6 IHC staining of human duodenum
    Figure 6 IHC staining of human duodenum
    (Immunohistochemical staining of human duodenum shows moderate cytoplasmic positivity in glandular cells.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2686/ihc_selected.jpg
    Figure 7 IF staining of human cell line U-251 MG
    Figure 7 IF staining of human cell line U-251 MG
    (Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & nuclear bodies.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/27280/258_B8_1_red_green.jpg
    Normal Tissue
    Figure 8 Cerebral cortex
    Figure 8 Cerebral cortex
    (Neuropil
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/28409/61378_B_8_5.jpg
    Figure 9 Adrenal gland
    Figure 9 Adrenal gland
    (Glandular cells
    Staining:High
    Intensity: Strong
    Quantity: 75%-25%
    Location: Cytoplasmic/membranous nuclear)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/28409/61378_B_5_5.jpg
    Figure 10 Colon
    Figure 10 Colon
    (Endothelial cells
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous
    Glandular cells
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous nuclear
    Peripheral nerve/ganglion
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/28409/61494_A_9_3.jpg
    Figure 11 Liver
    Figure 11 Liver
    (Hepatocytes
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous nuclear)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/28409/61494_A_9_4.jpg
    Figure 12 Kidney
    Figure 12 Kidney
    (Cells in glomeruli
    Staining:Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Location: Cytoplasmic/membranous nuclear
    Cells in tubules
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous nuclear)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/28409/61494_A_9_5.jpg
    Figure 13 Testis
    Figure 13 Testis
    (Spermatogonia cells
    Staining:Medium
    Intensity: Moderate
    Quantity: 75%-25%)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/28409/61494_A_6_6.jpg
    RNA Expression
    Figure 14 RNA cell line category: Low cell line specificity
    Figure 14 RNA cell line category: Low cell line specificity
    (Cell lines ordered by descending RNA expression order.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000097007-ABL1

    Specifications

    • Scaffold Name
    • 10-FnIII
    • Origin
    • Fibronectin
    • Core Structure
    • β-sandwich of 7 β-strands
    • Variable Regions
    • 2 Loops
    • Target
    • Abelson (Abl) kinase SH2 domain
    • Species Reactivity
    • Human
    • Expression Host
    • BL21 (DE3) cells
    • Affinity Constant
    • 7 nM
    • Applications
    • ELISA; immunohistochemistry; Microscopy; Flow Cytometer; Western Blotting; Functional Study

    Target

    • Alternative Names
    • Fibronectin; 10-FnIII; ABL; JTK7; p150; c-ABL; v-abl; c-ABL1; bcr/abl

    Related Resources

    • Related Signaling Pathways

    Downloads

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