10-FnIII scaffold protein anti-Human Abelson (Abl) kinase SH2 domain
CAT#: SFN-L001
The tenth fibronectin type III domain (10Fn3) that bound Abelson (Abl) kinase SH2 domain was isolated from the library using Phage display technology. The fibronectin type III domain (FN3) is a small autonomous folding unit which occurs in many animal proteins involving in ligand binding. The β-sandwich structure of FN3 closely resembles that of immunoglobulin domains. After affinity maturation, 10Fn3 variants were identified having affinities (KD=7 nM) for Abelson (Abl) kinase SH2 domain as determined by biosensor technology. It's potential to be used in diagnostic, research and therapeutic applications.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & nuclear bodies.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/27280/259_B8_2_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/28409/497_A9_2_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IHC staining of human heart muscle
Immunohistochemical staining of human heart muscle shows strong cytoplasmic positivity in myocytes.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/27251/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 4 IHC staining of human prostate
Immunohistochemical staining of human prostate shows cytoplasmic and membranous positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/27280/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 5 IHC staining of human adrenal gland
Immunohistochemical staining of human adrenal gland shows strong nuclear positivity in cortical cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/28409/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 6 IHC staining of human duodenum
Immunohistochemical staining of human duodenum shows moderate cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2686/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 7 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & nuclear bodies.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/27280/258_B8_1_red_green.jpg
Normal Tissue
Figure 8 Cerebral cortex
Neuropil
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/28409/61378_B_8_5.jpg
Normal Tissue
Figure 9 Adrenal gland
Glandular cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/28409/61378_B_5_5.jpg
Normal Tissue
Figure 10 Colon
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Peripheral nerve/ganglion
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/28409/61494_A_9_3.jpg
Normal Tissue
Figure 11 Liver
Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/28409/61494_A_9_4.jpg
Normal Tissue
Figure 12 Kidney
Cells in glomeruli
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/28409/61494_A_9_5.jpg
Normal Tissue
Figure 13 Testis
Spermatogonia cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/28409/61494_A_6_6.jpg
RNA Expression
Figure 14 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000097007-ABL1
❮
❯
Specifications
- Scaffold Name
- 10-FnIII
- Origin
- Fibronectin
- Core Structure
- β-sandwich of 7 β-strands
- Variable Regions
- 2 Loops
- Target
- Abelson (Abl) kinase SH2 domain
- Species Reactivity
- Human
- Expression Host
- BL21 (DE3) cells
- Affinity Constant
- 7 nM
- Applications
- ELISA; immunohistochemistry; Microscopy; Flow Cytometer; Western Blotting; Functional Study
Target
- Alternative Names
- Fibronectin; 10-FnIII; ABL; JTK7; p150; c-ABL; v-abl; c-ABL1; bcr/abl
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