APC-H-2Db/HPV16 E7 (RAHYNIVTF) MHC Tetramer
CAT#: MHC-LC368
This product is a tetramer of biotinylated peptide/MHC complex with streptavidin mainly composed of HPV16-derived peptide of RAHYNIVTF sequence covering 49-57 and H-2Db molecule. The pMHC tetramer recognizes mouse CD8 T cells, and can be used in the analysis of individual antigen-specific T cells.
Specifications
- Allele
- H-2Db
- Class
- Class I
- MHC Species
- Mouse
- Antigen
- HPV16 E7
- Antigen Species
- HPV16
- Peptide
- RAHYNIVTF
- Range
- 49-57
- Conjugate
- APC
- Application
- FCM
Target
- Antigen Introduction
- Papillomaviridae is an ancient taxonomic family of non-enveloped DNA viruses, collectively known as papillomaviruses. Several hundred species of papillomaviruses, traditionally referred to as "types", have been identified infecting all carefully inspected mammals, but also other amniotes such as birds, snakes and turtles. Infection by most papillomavirus types, depending on the type, is either asymptomatic (e.g. most Beta-PVs) or causes small benign tumors, known as papillomas or warts (e.g. human papillomavirus1, HPV6 or HPV11). Papillomas caused by some types, however, such as human papillomaviruses 16 and 18, carry a risk of becoming cancerous.
- Alternative Names
- HPV16 E7; Human papillomavirus type 16 E7
- Gene ID
- 1489079
- UniProt ID
- P03129
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Great for HPV16 E7 Research
This APC-labeled tetramer was very effective for identifying mouse CD8 T cells specific to the HPV16 E7 peptide RAHYNIVTF. The signal was bright and allowed for distinct separation of the target cell population.
Clear Flow Cytometry Staining
The H-2Db tetramer loaded with the 49-57 peptide sequence worked reliably in our assays. It helped us quantify antigen-specific T cells in our HPV research, providing consistent and high-quality data.
Q&As
-
Which HPV16 E7 epitope is recognized by this tetramer?
A: This tetramer recognizes the HPV16 E7 epitope with the sequence RAHYNIVTF (amino acids 49-57). It is presented by the mouse H-2Db MHC Class I molecule.
-
What is the conjugate on this MHC tetramer?
A: This product is conjugated with Allophycocyanin (APC). It is used in flow cytometry to analyze mouse CD8 T cells that are specific for the HPV16 E7 oncoprotein peptide.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
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Datasheet
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COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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