Recombinant Anti-HPV16 E7 Intrabody [Tat]
CAT#: IAB-B047(T)
Recombinant anti-HPV16 E7 intrabody in fusion with HIV Tat works within cells to bind HPV16 E7, expressed in 293T cells. The intrabody is specific for the E7 oncoprotein of HPV16 and is able to inhibit cell proliferation.
Specifications
- Immunogen
- Human papillomavirus16 transforming protein
- Host Species
- mouse
- Type
- scFv-Tat
- Cell Penetrating Peptide
- TAT
TAT was able to deliver different proteins, such as horseradish peroxidase and RNase A across cell membrane into the cytoplasm in different cell lines in vitro. The size range of proteins with effective delivery is from 30kDa to 120-150kDa. In one study, TAT-fused proteins are rapidly internalized by lipid raft−dependent macropinocytosis using a transducible TAT−Cre recombinase reporter assay on live cells. In another study, a TAT-fused protein was delivered into mitochondria of breast cancer cells and decreased the survival of breast cancer cells, which showed capability of TAT-fusion proteins to modulate mitochondrial function and cell survival. However, very few in vivo studies have succeeded. In one study, in vivo delivery of TAT- or penetratin-crosslinked Fab fragments yielded varied organ distributions and an overall increase in organ retention, which showed tissue localization.
- Species Reactivity
- HPV16
- Applications
- Formats of immunological tests: Immunofluorescence; Flow Cytometry; Western blot; Functional studies
Product Property
- Purity
- >95% as judged by SDS-polyacrylamide gel electrophoresis
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Target
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "HPV16 E7"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| IAB-B047(A) | Recombinant Anti-HPV16 E7 Intrabody [(D-Arg)9] | ICC, Neut, FuncS | scFv-(D-Arg)9 |
| IAB-B047(G) | Recombinant Anti-HPV16 E7 Intrabody [+36 GFP] | ELISA, WB, ICC, FuncS | scFv-(+36GFP) |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1064CQ | Recombinant Mouse Anti-HPV-16 E7 Antibody (289-17013(TVG-701Y)) | ELISA, FC, IHC-Fr, IP, WB | Mouse antibody |
| HPAB-2328LY | Rabbit Anti-HPV16 E7 Recombinant Antibody (HPAB-2328LY) | ELISA, ICC | Rabbit IgG |
| FAMAB-0191WJ | Mouse Anti-HPV16 E7 Recombinant Antibody (clone 69E2) | ICC, IF, IHC, ELISA, WB, Neut | Mouse IgG2a |
| ZG-0267J | Mouse Anti-HPV-16 E7 Recombinant Antibody (ZG-0267J) | WB, ELISA | Mouse IgG |
| ZG-0268J | Mouse Anti-HPV-16 E7 Recombinant Antibody (ZG-0268J) | WB, IHC, ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-543LC | Anti-HPV16 E7 Recombinant Antibody (6C10) | ELISA | Mouse antibody |
| MRO-546LC | Anti-HPV16 E7 Recombinant Antibody | ELISA | Rabbit antibody |
| MRO-552LC | Anti-HPV16 E7 Recombinant Antibody (8E5) | ELISA | Mouse antibody |
| MRO-553LC | Anti-HPV16 E7 Recombinant Antibody (8C10) | ELISA | Mouse antibody |
| MRO-554LC | Anti-HPV16 E7 Recombinant Antibody (8G9) | ELISA | Mouse antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC750 | A*0201/HPV16 E7 (YMLDLQPETT) MHC Tetramer | FCM | |
| MHC-LC751 | A*0201/HPV16 E7 (DLLLGTLNI) MHC Tetramer | FCM | |
| MHC-LC752 | A*0201/HPV16 E7 (LLLGTLNIV) MHC Tetramer | FCM | |
| MHC-LC753 | A*0201/HPV16 E7 (LQPPDPVGL) MHC Tetramer | FCM | |
| MHC-LC754 | A*0201/HPV16 E7 (PLTQHYQIL) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-2328LY-F(E) | Rabbit Anti-HPV16 E7 Recombinant Antibody; Fab Fragment (HPAB-2328LY-F(E)) | ELISA, ICC | Rabbit Fab |
| FAMAB-0191WJ-F(E) | Mouse Anti-HPV16 E7 Recombinant Antibody (clone 69E2); Fab Fragment | ICC, IF, IHC, ELISA, WB, Neut | Mouse Fab |
| FAMAB-0192WJ-F(E) | Mouse Anti-HPV16 E7 Recombinant Antibody (clone 79A11); Fab Fragment | ICC, IF, IHC, ELISA, WB, Neut | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-2328LY-S(P) | Rabbit Anti-HPV16 E7 Recombinant Antibody; scFv Fragment (HPAB-2328LY-S(P)) | ELISA, ICC | Rabbit scFv |
| FAMAB-0191WJ-S(P) | Mouse Anti-HPV16 E7 Recombinant Antibody (clone 69E2); scFv Fragment | ICC, IF, IHC, ELISA, WB, Neut | Mouse scFv |
| FAMAB-0192WJ-S(P) | Mouse Anti-HPV16 E7 Recombinant Antibody (clone 79A11); scFv Fragment | ICC, IF, IHC, ELISA, WB, Neut | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0192WJ | Mouse Anti-HPV16 E7 Recombinant Antibody (clone 79A11) | ICC, IF, IHC, ELISA, WB, Neut | Mouse IgM |
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