Anti-Human ACE Recombinant Antibody (9B9) (CAT#: TAB-0635CLV)

Figure 1 mAb-induced ACE shedding.

CHO-ACE cells were incubated with different anti-ACE mAbs (10 µg/ml) at 4 °C in a binding assay (A, cell ELISA) and at 37 °C in an assay of ACE release (B) for 4 h. (C) The ratio of shedding to binding for each mAb.

Balyasnikova, I. V., Karran, E. H., Albrecht, R. F., & Danilov, S. M. (2002). Epitope-specific antibody-induced cleavage of angiotensin-converting enzyme from the cell surface. Biochemical Journal, 362(3), 585-595.

Figure 2 Effect of low temperature and 1,10-phenanthroline on basal and antibody-induced ACE shedding.

(A) CHO-ACE cells were incubated in the presence of mAbs to ACE (10 µg/ml) at 4 or 37 °C for 4 h (n¯3). (B) CHO-ACE cells were pre-incubated with 1,10-phenanthroline (5 mM) during 30 min at 37 °C, after which mAbs to ACE (10 µg/ml) were added for the subsequent 4 h. ACE release was estimated by measuring ACE activity in the culture fluid (n¯3). (C) The mAb binding in the presence of 1,10-phenanthroline was estimated by cell ELISA, as described in the Materials and methods section (n¯3). mIgG, non-immune mouse IgG.

Balyasnikova, I. V., Karran, E. H., Albrecht, R. F., & Danilov, S. M. (2002). Epitope-specific antibody-induced cleavage of angiotensin-converting enzyme from the cell surface. Biochemical Journal, 362(3), 585-595.