Human Anti-AGR2 Recombinant Antibody (TAB-0708CLV) (CAT#: TAB-0708CLV)
This product is a recombinant Human antibody that recognizes AGR2. The antibody was expressed in mammalian cells with chemically defined culture media and was purified by affinity chromatography.
Specific Inquiry
DB
Figure 1 Anti-AGR2 Recombinant Antibody (TAB-0708CLV) in DB
Dot Blot analysis of TAB-0708CLV was performed by coating with human AGR2 protein (His tag).
TAB-0708CLV incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-Human IgG as a secondary antibody (1: 2000)
WB
Figure 2 Anti-AGR2 Recombinant Antibody (TAB-0708CLV) in WB
Western blot analysis of TAB-0708CLV was performed by loading human AGR2 protein (His tag) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with TAB-0708CLV and HRP goat anti-Human IgG as a secondary antibody (1: 2000). Chemiluminescent detection was performed.
Lane 1: Reduced antigen (0.1 μg)
Lane 2: Reduced antigen (0.3 μg)
Lane 3: Reduced antigen (0.6 μg)
ELISA
Figure 3 Anti-AGR2 Recombinant Antibody (TAB-0708CLV) in ELISA
ELISA analysis of TAB-0708CLV was performed by coating with human AGR2 protein (His tag). Then blocked with BSA and incubated with TAB-0708CLV. The HRP-conjugated goat anti-Human IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
DB
Figure 4 Anti-AGR2 Recombinant Antibody (TAB-0708CLV) in DB
Dot Blot analysis of TAB-0708CLV was performed by coating with mouse AGR2 protein (His tag).
TAB-0708CLV incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-Human IgG as a secondary antibody (1: 2000)
WB
Figure 5 Anti-AGR2 Recombinant Antibody (TAB-0708CLV) in WB
Western blot analysis of TAB-0708CLV was performed by loading mouse AGR2 protein (His tag) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with TAB-0708CLV and HRP goat anti-Human IgG as a secondary antibody (1: 2000). Chemiluminescent detection was performed.
Lane 1: Reduced antigen (0.1 μg)
Lane 2: Reduced antigen (0.3 μg)
Lane 3: Reduced antigen (0.6 μg)
ELISA
Figure 6 Anti-AGR2 Recombinant Antibody (TAB-0708CLV) in ELISA
ELISA analysis of TAB-0708CLV was performed by coating with mouse AGR2 protein (His tag). Then blocked with BSA and incubated with TAB-0708CLV. The HRP-conjugated goat anti-Human IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Specifications
- Host Species
- Human
- Type
- Humanized IgG1, κ
- Specificity
- Human AGR2
- Species Reactivity
- Cross-react with murine AGR2
- Applications
- Inhib, FC
- Related Disease
- Tumor
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- AGR2; anterior gradient 2, protein disulphide isomerase family member; AG2; GOB-4; HAG-2; XAG-2; PDIA17; HEL-S-11
- Gene ID
- 10551
- UniProt ID
- O95994
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Non-Human Primate (NHP) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-130-VHH | Recombinant Anti-Human AGR2 VHH Single Domain Antibody | IHC, IP, FC, Neut, FUNC | Llama VHH |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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