Hi-Affi™ Rabbit Anti-ARID1A Recombinant Antibody (clone DS248AB)
CAT#: MOR-0248
Recombinant Rabbit anti-ARID1A monoclonal antibody for IHC-P, ICC/IF, excellent for producing superior results. Hi-Affi™ antibodies are developed from different sources, such as B cells of immunized animals, hybridoma, phage display library. The application of recombinant technology avoids the traditional ascitic fluid approach, which achieves the antibody generation in a humane way with much larger scale, greater specificity and sensitivity, and not susceptible to cell-line drift or lot-to-lot variation.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5456/6_F12_1_selected.jpg
Normal Tissue
Figure 2 IHC staining of human nasopharynx
Immunohistochemical staining of human nasopharynx shows strong nuclear positivity.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5456/ihc_selected.jpg
Normal Tissue
Figure 3 IHC staining of human prostate
Immunohistochemical staining of human prostate shows strong nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16334/ihc_selected.jpg
Normal Tissue
Figure 4 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5456/19571_A_8_8.jpg
Normal Tissue
Figure 5 Cerebral cortex
Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5456/19571_B_9_5.jpg
Normal Tissue
Figure 6 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5456/19571_A_7_3.jpg
Normal Tissue
Figure 7 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5456/19571_A_7_5.jpg
Normal Tissue
Figure 8 Testis
Sertoli cells Staining: Medium Intensity: Moderate Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5456/19571_A_6_6.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000117713-ARID1A
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Specifications
- Immunogen
- Recombinant fragment within Human ARID1A aa 1200-1350. The exact sequence is proprietary.
- Host Species
- Rabbit
- Derivation
- Phage display library screening
- Type
- Rabbit IgG
- Species Reactivity
- Mouse, Rat, Human
- Clone
- DS248AB
- Applications
- IHC-P, IF
- Conjugate
- Unconjugated
Product Property
- Purification
- Purified with Absolute Mag™ Protein A Agarose
- Purity
- >95% as determined by analysis by SDS-PAGE
- Format
- Liquid
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- AT Rich Interactive Domain 1A (SWI-Like); SWI/SNF-Related, Matrix-Associated, Actin-Dependent Regulator Of Chromatin Subfamily F Member 1; ARID Domain-Containing Protein 1A; SWI/SNF Complex Protein P270; BRG1-Associated Factor 250a; SWI-Like Protein; Osa Homolog 1; BAF250a; SMARCF1; C1orf4; BAF250; HOSA1; B120; OSA1; HELD; SWI/SNF Related, Matrix Associated, Actin Dependent Regulator Of Chromatin, Subfamily F, Member 1; AT Rich Interactive Domain 1A (SWI- Like); Chromatin Remodeling Factor P250; BRG1-Associated Factor 250; OSA1 Nuclear Protein; Brain Protein 120; BM029; MRD14; P270; ELD
- Gene ID
- 8289
- UniProt ID
- O14497
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "ARID1A"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3992z | Mouse Anti-ARID1A Recombinant Antibody (clone 30B11) | WB, IP, IF, ELISA | Mouse IgG1, κ |
| MOB-0710MZ | Mouse Anti-ARID1A Recombinant Antibody (clone 3046D6b) | WB | Mouse IgG1 |
| MRO-0120-CN | Rabbit Anti-ARID1A Recombinant Antibody (clone CBACN-042) | IF, IHC | Rabbit IgG |
| VS3-CJ122 | Rabbit Anti-ARID1A Recombinant Antibody (VS3-CJ122) | ICC, IF, IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY166 | Anti-ARID1A Immunohistochemistry Kit | IHC | |
| VS-0525-XY514 | Anti-Mouse ARID1A Immunohistochemistry Kit | IHC | |
| VS-0525-XY513 | Anti-Human ARID1A Immunohistochemistry Kit | IHC |
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