Mouse Anti-ARID1A Recombinant Antibody (clone 30B11)
CAT#: MOB-3992z
This product is a mouse antibody that recognizes human ARID1A. It can be used in the following applications: WB, IP, IF, ELISA.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5456/6_F12_1_selected.jpg
Normal Tissue
Figure 2 IHC staining of human nasopharynx
Immunohistochemical staining of human nasopharynx shows strong nuclear positivity.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5456/ihc_selected.jpg
Normal Tissue
Figure 3 IHC staining of human prostate
Immunohistochemical staining of human prostate shows strong nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16334/ihc_selected.jpg
Normal Tissue
Figure 4 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5456/19571_A_8_8.jpg
Normal Tissue
Figure 5 Cerebral cortex
Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5456/19571_B_9_5.jpg
Normal Tissue
Figure 6 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5456/19571_A_7_3.jpg
Normal Tissue
Figure 7 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5456/19571_A_7_5.jpg
Normal Tissue
Figure 8 Testis
Sertoli cells Staining: Medium Intensity: Moderate Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5456/19571_A_6_6.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000117713-ARID1A
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Specifications
- Immunogen
- Human AT rich interactive domain 1A (SWI-like)
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- Mouse IgG1, κ
- Specificity
- Human ARID1A
- Species Reactivity
- Human
- Clone
- 30B11
- Applications
- WB, IP, IF, ELISA
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Western Blot, Immunoprecipitation Assay, Immunofluorescence Assay, Enzyme-linked Immunosorbent Assay.
Target
- Alternative Names
- ARID1A; BRG1-Associated Factor 250a; B120; P270; ELD; SWI/SNF Related, Matrix Associated, Actin Dependent Regulator Of Chromatin, Subfamily F, Member 1; Chromatin Remodeling Factor P250; AT-Rich Interactive Domain-Containing Protein 1A; ARID Domain-Contai
- UniProt ID
- O14497
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Versatile For Chromatin Studies
Clone 30B11 is a fantastic antibody for ARID1A. We have successfully used it in Western Blot, IP, and Immunofluorescence. It specifically recognizes the region between amino acids 1236-1325, allowing us to study the SWI/SNF complex and chromatin remodeling effectively.
Strong Signal In ELISA
We incorporated this mouse antibody into our ELISA protocols to detect human ARID1A. It provided a strong and linear signal. The IgG1 kappa isotype works well with our secondary antibodies, and the SDS-PAGE data confirms the high purity of the product.
Q&As
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What immunogen region does this antibody recognize?
A: The antibody (Clone 30B11) recognizes Human ARID1A. It was raised against an immunogen corresponding to amino acids 1236-1325 of the protein, which is part of the SWI/SNF chromatin remodeling complex.
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What are the reported applications for this product?
A: This mouse antibody has been reported for use in multiple applications, including Western Blot (WB), Immunoprecipitation (IP), Immunofluorescence (IF), and Enzyme-linked Immunosorbent Assay (ELISA).
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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