Hi-Affi™ Rabbit Anti-ARID3A Recombinant Antibody (clone DS249AB) (CAT#: MOR-0249)
Recombinant Rabbit anti-ARID3A monoclonal antibody for ICC/IF, IHC-P, WB, excellent for producing superior results. Hi-Affi™ antibodies are developed from different sources, such as B cells of immunized animals, hybridoma, phage display library. The application of recombinant technology avoids the traditional ascitic fluid approach, which achieves the antibody generation in a humane way with much larger scale, greater specificity and sensitivity, and not susceptible to cell-line drift or lot-to-lot variation.
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Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line CACO-2
(Immunofluorescent staining of human cell line CACO-2 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/76330/1832_F6_1_selected.jpg
(Immunofluorescent staining of human cell line CACO-2 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/76330/1832_F6_1_selected.jpg
Figure 2 IF staining of human cell line K-562
(Immunofluorescent staining of human cell line K-562 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/76330/1892_C8_1_red_green.jpg
(Immunofluorescent staining of human cell line K-562 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/76330/1892_C8_1_red_green.jpg
Figure 3 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/76330/1890_A10_1_cr5b9b86ed1d259_red_green.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/76330/1890_A10_1_cr5b9b86ed1d259_red_green.jpg
Figure 4 Placenta
(Cytotrophoblasts
Staining:High
Intensity: Strong
Quantity: 75%-25%
Hofbauer cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Syncytiotrophoblasts - cell body
Staining:High
Intensity: Strong
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/76330/165564_A_1_7.jpg
(Cytotrophoblasts
Staining:High
Intensity: Strong
Quantity: 75%-25%
Hofbauer cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Syncytiotrophoblasts - cell body
Staining:High
Intensity: Strong
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/76330/165564_A_1_7.jpg
Figure 5 Testis
(Cells in seminiferous ducts
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/76330/165564_A_6_6.jpg
(Cells in seminiferous ducts
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/76330/165564_A_6_6.jpg
Figure 6 RNA cell line category: Cell line enhanced (BEWO, CACO-2, Hep G2, K-562, THP-1)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000116017-ARID3A
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000116017-ARID3A
Specifications
- Immunogen
- Synthetic peptide, corresponding to residues in Human DRIL1.
- Host Species
- Rabbit
- Derivation
- Phage display library screening
- Type
- Rabbit IgG
- Species Reactivity
- Mouse, Rat, Human
- Clone
- DS249AB
- Applications
- IF, IHC-P, WB
- Conjugate
- Unconjugated
Product Property
- Purification
- Purified with Absolute Mag™ Protein A Agarose
- Purity
- >95% as determined by analysis by SDS-PAGE
- Format
- Liquid
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- AT Rich Interactive Domain 3A (BRIGHT-Like); B-Cell Regulator Of IgH Transcription; ARID Domain-Containing Protein 3A; Dead Ringer-Like Protein 1; E2F-Binding Protein 1; BRIGHT; E2FBP1; DRIL1; DRIL3; AT Rich Interactive Domain 3A (BRIGHT- Like) Protein; AT Rich Interactive Domain 3A (BRIGHT- Like); Dead Ringer-Like 1 (Drosophila); ARID Domain-Containing 3A; Dead Ringer-Like 1; DRX
- Gene ID
- 1820
- UniProt ID
- Q99856
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-207 | Human Anti-ARID3A Recombinant Antibody (clone U46) | IP, IF, IP-MS | Human IgG1 |
| MOB-2077MZ | Mouse Anti-ARID3A Recombinant Antibody (clone BSJEF0D22) | WB | Mouse IgG1 |
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For Research Use Only. Not For Clinical Use.
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