Hi-Affi™ Rabbit Anti-ARID3A Recombinant Antibody (clone DS249AB)
CAT#: MOR-0249
Recombinant Rabbit anti-ARID3A monoclonal antibody for ICC/IF, IHC-P, WB, excellent for producing superior results. Hi-Affi™ antibodies are developed from different sources, such as B cells of immunized animals, hybridoma, phage display library. The application of recombinant technology avoids the traditional ascitic fluid approach, which achieves the antibody generation in a humane way with much larger scale, greater specificity and sensitivity, and not susceptible to cell-line drift or lot-to-lot variation.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line CACO-2
Immunofluorescent staining of human cell line CACO-2 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/76330/1832_F6_1_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line K-562
Immunofluorescent staining of human cell line K-562 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/76330/1892_C8_1_red_green.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/76330/1890_A10_1_cr5b9b86ed1d259_red_green.jpg
Normal Tissue
Figure 4 Placenta
Cytotrophoblasts
Staining:High
Intensity: Strong
Quantity: 75%-25%
Hofbauer cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Syncytiotrophoblasts - cell body
Staining:High
Intensity: Strong
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/76330/165564_A_1_7.jpg
Normal Tissue
Figure 5 Testis
Cells in seminiferous ducts
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/76330/165564_A_6_6.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (BEWO, CACO-2, Hep G2, K-562, THP-1)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000116017-ARID3A
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Specifications
- Immunogen
- Synthetic peptide, corresponding to residues in Human DRIL1.
- Host Species
- Rabbit
- Derivation
- Phage display library screening
- Type
- Rabbit IgG
- Species Reactivity
- Mouse, Rat, Human
- Clone
- DS249AB
- Applications
- IF, IHC-P, WB
- Conjugate
- Unconjugated
Product Property
- Purification
- Purified with Absolute Mag™ Protein A Agarose
- Purity
- >95% as determined by analysis by SDS-PAGE
- Format
- Liquid
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- AT Rich Interactive Domain 3A (BRIGHT-Like); B-Cell Regulator Of IgH Transcription; ARID Domain-Containing Protein 3A; Dead Ringer-Like Protein 1; E2F-Binding Protein 1; BRIGHT; E2FBP1; DRIL1; DRIL3; AT Rich Interactive Domain 3A (BRIGHT- Like) Protein; AT Rich Interactive Domain 3A (BRIGHT- Like); Dead Ringer-Like 1 (Drosophila); ARID Domain-Containing 3A; Dead Ringer-Like 1; DRX
- Gene ID
- 1820
- UniProt ID
- Q99856
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "ARID3A"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-207 | Human Anti-ARID3A Recombinant Antibody (clone U46) | IP, IF, IP-MS | Human IgG1 |
| MOB-2077MZ | Mouse Anti-ARID3A Recombinant Antibody (clone BSJEF0D22) | WB | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-209 | Human Anti-ARID3A Recombinant Antibody (clone U5); Fab Fragment | IF, IP-MS | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC1970 | PE-B7/Human ARID3A (SPKLPVSSL) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY19 | AbPlus™ Anti-ARID3A Magnetic Beads (U46) | IP, IP-MS, Protein Purification | |
| VS-0724-YC519 | AbPlus™ Anti-ARID3A Magnetic Beads (VS-0724-YC519) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY167 | Anti-ARID3A Immunohistochemistry Kit | IHC | |
| VS-0525-XY518 | Anti-Human ARID3A Immunohistochemistry Kit | IHC |
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