Recombinant Mouse Antibody ( LA-2) is capable of binding to B. burgdorferi OspA, expressed in Chinese Hamster Ovary cells (CHO). This murine monoclonal antibody defines an important protective B-cell epitope of OspA against which protective sera have strong levels of reactivity.
Figure 1 Western blot analysis of B. burgdorferi-specific antibodies.
(A) mAbs, IS, and NMS used for reconstitution of scid mice, tested on strains B31 and ZS7 of B. burgdorferi. (B) Sera from individual scid mice reconstituted with the respective mAbs, IS, or NMS, tested on strain ZS7 (strain used for infection of mice) and on strain B31. The following mAbs or sera were used: mAb LA-5 (lane 1), mAb LA-2 (lane 2), mAb LA-10 (lane 3), mAb LA-21 (lane 4), anti-B31 IS (lane 5), anti-ZS7 IS (lane 6) and NMS (lane 7). (C) Amount (Ag/ml) of B. burgdorferi-specific antibody(ies) in reconstituted scid mice during the observation period (one representative experiment). Curves: 1, mAb LA-10; 2, mAb LA-21; 3, mAb LA-2; 4, mAb LA-5; 5, anti-B31; 6, PBS.
Schaible, U. E., Kramer, M. D., Eichmann, K., Modolell, M., Museteanu, C., & Simon, M. M. (1990). Monoclonal antibodies specific for the outer surface protein A (OspA) of Borrelia burgdorferi prevent Lyme borreliosis in severe combined immunodeficiency (scid) mice. Proceedings of the National Academy of Sciences, 87(10), 3768-3772.
Figure 2 Histopathology in scid mice simultaneously inoculated s.c. with B. burgdorferi and i.p. with mAb LA-5 (a and c) or mAb LA-2 (b and d).
(a) Tibiotarsal joint of a scid mouse treated with LA-5. (b) Tibiotarsal joint of a scid mouse treated with the mAb LA-2. (c) Heart of a scid mouse treated with mAb LA-5. (d) Heart of a scid mouse treated with mAb LA-2.
Schaible, U. E., Kramer, M. D., Eichmann, K., Modolell, M., Museteanu, C., & Simon, M. M. (1990). Monoclonal antibodies specific for the outer surface protein A (OspA) of Borrelia burgdorferi prevent Lyme borreliosis in severe combined immunodeficiency (scid) mice. Proceedings of the National Academy of Sciences, 87(10), 3768-3772.
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CAT | Product Name | Application | Type |
---|---|---|---|
PABC-012 | Recombinant Mouse Anti-B. burgdorferi OspA Antibody (mAb184.1) | Neut | IgG |
MRO-017CQ | Recombinant Mouse Anti-B. burgdorferi OspA Antibody (0551) | ELISA, IF | IgG |
FAMAB-0187JF | Human Anti-B. burgdorferi OspA Recombinant Antibody (clone 3-24) | WB, ELISA, IHC | Human IgG |
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(Creative Biolabs Cat# PABZ-012, RRID: AB_3111705)
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