Mouse Anti-BRDU Recombinant Antibody (clone BMG-6H8); Fab Fragment (CAT#: DrMAB-188)

Recombinant Mouse Antibody binds selectively to Mouse BrdU.

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IHC

Figure 1 Immunohistochemical analysis of BrdU retention in keratinocytes at various time points after labeling.

Albert, M. R., Foster, R. A., & Vogel, J. C. (2001). Murine epidermal label-retaining cells isolated by flow cytometry do not express the stem cell markers CD34, Sca-1, or Flk-1. Journal of investigative dermatology, 117(4), 943-948.

FC

Figure 2 Flow cytometric analysis of cells double stained for BrdU and stem cell markers.

Albert, M. R., Foster, R. A., & Vogel, J. C. (2001). Murine epidermal label-retaining cells isolated by flow cytometry do not express the stem cell markers CD34, Sca-1, or Flk-1. Journal of investigative dermatology, 117(4), 943-948.


Specifications

  • Immunogen
  • The details of the immunogen for this antibody are not available.
  • Host Species
  • Mouse
  • Type
  • Mouse Fab
  • Specificity
  • The antibody reacts with the thymidine analogue 5-bromo-2'-deoxyuridine (BrdU) and with cellular DNA that contains incorporated BrdU; it shows no crossreactivity with other cellular components. The antibody may be applied for the qualitative measurement of cell proliferation (BrdU incorporation) on a single-cell level, and for quantitative measurement in a cell population by ELISA.
  • Clone
  • BMG-6H8
  • Applications
  • ELISA, ICC, IHC, FC
  • Conjugate
  • HRP

Applications

  • Application Notes
  • The BrdU antibody has been reported in applications of Enzyme-linked Immunosorbent Assay, Immunocytochemistry, Immunohistochemistry, Flow Cytometry.

Target

  • Alternative Names
  • 5-BROMO-1-(2-DEOXY-BETA-D-RIBOFURANOSYL)URACIL; 5-BRDU; 5-BROMO DEOXYURIDINE; 5-BROMO-2"-DESOXYURIDINE; (+)-5-BROMO-2"-DEOXYURIDINE; 5-BROMO-2"-DEOXYURIDINE; 2"-DEOXY-5-BROMOROURIDINE; 2"-DEOXY-5-BROMOURIDINE; BUDR; BROMO2"-DEOXYURIDINE,5-; BRUDR; BROXURI

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

* Abbreviations
3D IHC3D Immunohistochemistry
ActivActivation
AgonistAgonist
ApopApoptosis
BABioassay
BIBioimaging
BlockBlocking
Cell ScreeningCell Screening
SeparationCell Separation
ChIPChromatin Immunoprecipitation
CMCDComplement Mediated Cell Depletion
CostimCostimulation
CytCytotoxicity
DepletionDepletion
DBDot Blot
EMElectron Microscopy
ELISAEnzyme-linked Immunosorbent Assay
ELISPOTEnzyme-linked Immunosorbent Spot
FCFlow Cytometry
FuncSFunctional Assay
GSGel Super Shift Assay
HAHemagglutination
IAImmunoassay
IBImmunoblotting
ICCImmunocytochemistry
IDImmunodiffusion
IFImmunofluorescence
IHCImmunohistochemistry
IHC-FrImmunohistochemistry-Frozen
IHC-PImmunohistochemistry-Paraffin
REImmunohistology - Resin Sections
IPImmunoprecipitation
IRMAImmunoradiometric Assay
SHIn situ hybridization
InhibInhibition
ICFCIntracellular Staining for Flow Cytometry
KO/KD-WBKnockout/Knockdown target confirmation by Western Blot
Live cell imagingLive cell imaging
CyTOF®Mass Cytometry
MeDIPMethylated DNA Immunoprecipitation
MultiplexMultiplex bead-based assay
NeutNeutralization
PPProtein Purification
PGProteogenomics
RIRadial Immunodiffusion
RIARadioimmunoassay
StimStimulation
SPRSurface Plasmon Resonance
TCTissue Culture
TBTurbidimetry
WBWestern Blot

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