This product is a human antibody that specifically recognizes botA from C. botulinum. The antibody 3F10 is an epitope-specific antibody that can be used in ELISA and other immunological assays.
Figure 1 Recombinant Human Anti-C. botulinum botA Antibody (3F10) (EPAF-0108CQ) in SDS-PAGE
SDS-PAGE analysis of EPAF-0108CQ in non-reduced (Lane 2) and reduced (Lane 1) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.
Figure 2 Recombinant Human Anti-C. botulinum botA Antibody (3F10) (EPAF-0108CQ) in WB
Western blot analysis of EPAF-0108CQ was performed by loading Recombinant Clostridium botulinum Botulinum neurotoxin type A(botA) (expressed in E. coli, his Tag).
EPAF-0108CQ incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Goat Anti-Human IgG (H+L)
Lane 1: Reducing Antigen (0.1 μg)
Lane 2: Reducing Antigen (0.3 μg)
Lane 3: Reducing Antigen (0.6 μg)
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Download resources about recombinant antibody development and antibody engineering to boost your research.
CAT | Product Name | Application | Type |
---|---|---|---|
EPAF-0042LC | Recombinant Mouse Anti-C. botulinum botA Antibody (HuC25) | FC | IgG |
EPAF-0043LC | Recombinant Mouse Anti-C. botulinum botA Antibody (S25) | FC | IgG |
EPAF-0044LC | Recombinant Mouse Anti-C. botulinum botA Antibody (C25) | ELISA, FuncS | IgG |
EPAF-0045LC | Recombinant Human Anti-C. botulinum botA Antibody (3D12) | ELISA, FuncS | IgG |
EPAF-0106CQ | Recombinant Mouse Anti-C. botulinum botA Antibody (1C6) | ELISA | IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
PABC-579 | Recombinant Alpaca Anti-C. botulinum botA Single Domain Antibody (VHH-C2) | Neut | Alpaca VHH |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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