Recombinant Anti-human CCNE1 Intrabody [(D-Arg)9]
CAT#: IAB-B078(A)
Recombinant anti-CCNE1 intrabody linked to (D-Arg)9 works within cells to bind CCNE1, expressed in E. coli cells. Anti-cyclin E single-chain antibodiess (sFvs) are used to block cyclin E function in normal and breast cancer cells.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18169/if_selected.jpg
Normal Tissue
Figure 2 Testis
Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: nuclear Leydig cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18169/43894_A_4_6.jpg
Normal Tissue
Figure 3 Placenta
Decidual cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/308/2153_A_1_7.jpg
Normal Tissue
Figure 4 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Glial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18169/43894_B_7_5.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16682/36096_A_7_3.jpg
Normal Tissue
Figure 6 Liver
Hepatocytes Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18169/43894_A_9_4.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (BEWO)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000105173-CCNE1
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Specifications
- Immunogen
- human cyclin E1
- Host Species
- mouse
- Type
- scFv-(D-Arg)9
- Cell Penetrating Peptide
- (D-Arg)9
It has been proven that polyarginine peptides composed of nine arginine amino acids (Arg-9) are able to translocate across cells very efficiently. This raises the question if molecular dynamics simulations would show a similar pathway for this highly hydrophilic and charged peptide as predicted for the Tat peptide. The simplicity of Arg-9 over other CPPs also helps isolate the role of the Arg sidechain on the translocation without the effect of other sidechains. Therefore, in this work we study computationally the translocation of Arg-9 through a lipid bilayer and show that the translocation mechanism is the same as for the Tat peptide.
- Species Reactivity
- human
- Applications
- Can be useful in applications such as: Fluorescence-activated cell sorting; Immunocytochemistry; Functional studies
Product Property
- Purity
- >90% assessed by SDS-PAGE
- Storage
- 4 °C, -20°C if preferred
Target
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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COA
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See other products for "CCNE1"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| IAB-B078(G) | Recombinant Anti-human CCNE1 Intrabody [+36 GFP] | PCA, WB, FuncS | scFv-Tat |
| IAB-B078(T) | Recombinant Anti-human CCNE1 Intrabody [Tat] | FACS, ELISA, IF, FuncS | scFv-Tat |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0112CT | Mouse Anti-CCNE1 Recombinant Antibody (clone 0.U.307) | ELISA | Mouse IgG |
| VS3-XY184 | Mouse Anti-CCNE1 Recombinant Antibody (clone 5F8B4) | WB, IHC, FC | Mouse IgG1 |
| VS3-XY185 | Mouse Anti-CCNE1 Recombinant Antibody (clone 5F8C5) | ELISA, WB, FC | Mouse IgG1 |
| VS3-XY186 | Mouse Anti-CCNE1 Recombinant Antibody (clone 4A8) | ELISA, WB, FC | Mouse IgG1 |
| VS3-WK2145 | Rabbit Anti-CCNE1 (pT77) Recombinant Antibody (clone R02-6U7) | WB, IHC-P, ICC, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0517 | Hi-Affi™ Rabbit Anti-CCNE1 Recombinant Antibody (clone DS517AB) | FC, IF, IP, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1090 | Anti-CCNE1 Immunohistochemistry Kit | IHC |
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