Recombinant Anti-human CCNE1 Intrabody [(D-Arg)9] (CAT#: IAB-B078(A))
Recombinant anti-CCNE1 intrabody linked to (D-Arg)9 works within cells to bind CCNE1, expressed in E. coli cells. Anti-cyclin E single-chain antibodiess (sFvs) are used to block cyclin E function in normal and breast cancer cells.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18169/if_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18169/if_selected.jpg
Figure 2 Testis
(Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: nuclear Leydig cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18169/43894_A_4_6.jpg
(Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: nuclear Leydig cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18169/43894_A_4_6.jpg
Figure 3 Placenta
(Decidual cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/308/2153_A_1_7.jpg
(Decidual cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/308/2153_A_1_7.jpg
Figure 4 Cerebral cortex
(Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Glial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18169/43894_B_7_5.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Glial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18169/43894_B_7_5.jpg
Figure 5 Colon
(Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/16682/36096_A_7_3.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/16682/36096_A_7_3.jpg
Figure 6 Liver
(Hepatocytes Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18169/43894_A_9_4.jpg
(Hepatocytes Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18169/43894_A_9_4.jpg
Figure 7 RNA cell line category: Cell line enhanced (BEWO)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000105173-CCNE1
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000105173-CCNE1
Specifications
- Immunogen
- human cyclin E1
- Host Species
- mouse
- Type
- scFv-(D-Arg)9
- Cell Penetrating Peptide
- (D-Arg)9
It has been proven that polyarginine peptides composed of nine arginine amino acids (Arg-9) are able to translocate across cells very efficiently. This raises the question if molecular dynamics simulations would show a similar pathway for this highly hydrophilic and charged peptide as predicted for the Tat peptide. The simplicity of Arg-9 over other CPPs also helps isolate the role of the Arg sidechain on the translocation without the effect of other sidechains. Therefore, in this work we study computationally the translocation of Arg-9 through a lipid bilayer and show that the translocation mechanism is the same as for the Tat peptide.
- Species Reactivity
- human
- Applications
- Can be useful in applications such as: Fluorescence-activated cell sorting; Immunocytochemistry; Functional studies
Product Property
- Purity
- >90% assessed by SDS-PAGE
- Storage
- 4 °C, -20°C if preferred
Target
- Alternative Names
- CCNE1; cyclin E1; CCNE; G1/S-specific cyclin-E1; cyclin Es; cyclin Et; cyclin E variant ex5del; cyclin E variant ex7del
- Gene ID
- 898
- UniProt ID
- P24864
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| IAB-B078(G) | Recombinant Anti-human CCNE1 Intrabody [+36 GFP] | PCA, WB, FuncS | scFv-Tat |
| IAB-B078(T) | Recombinant Anti-human CCNE1 Intrabody [Tat] | FACS, ELISA, IF, FuncS | scFv-Tat |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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