Chimeric (Mouse/Human) Anti-CD20 Recombinant Antibody (TP-082CL)

Customer Review

There are currently no Customer reviews or questions for TP-082CL. Click the button above to contact us or submit your feedback about this product.

David Walker
09.Sep,23

Excellent for Rituximab Studies

The Rituximab has been excellent for our CD20 studies. Its specificity and sensitivity are top-notch, providing clear and reliable results. This antibody has significantly improved the accuracy of our experiments and saved us a lot of troubleshooting time.

Emily Turner
11.Oct,22

Reliable in Immunoassays

We incorporated this antibody into our immunoassays, and it has been a great asset. The specificity for CD20 is precise, allowing for accurate detection and analysis. The reliable performance has made it an invaluable tool in our lab.

John Harris
20.Jul,21

Consistent and High-Quality

This antibody has proven to be consistent and high-quality in our assays. The reproducible results and strong affinity for CD20 have significantly improved our experiments, making it a crucial part of our research toolkit. The consistent performance has greatly enhanced our data quality.

Q&As

1. Is Rituximab suitable for use in flow cytometry analysis?

   A: Yes, Rituximab (TP-082CL) is suitable for use in flow cytometry analysis. It provides specific binding to CD20, allowing for the detection of CD20-expressing cells in flow cytometry assays.

2. What are the storage recommendations for Rituximab?

   A: The recommended storage condition for Rituximab (TP-082CL) is at -20°C or lower. For short-term storage, it can be kept at 2-8°C. To ensure stability, avoid repeated freeze-thaw cycles.

3. Can Rituximab be used in immunoprecipitation assays?

   A: Yes, Rituximab (TP-082CL) can be used in immunoprecipitation assays. It provides specific binding to CD20, enabling the successful precipitation of the target protein from complex mixtures.

4. Is Rituximab effective in ELISA applications?

   A: Yes, Rituximab (TP-082CL) is effective in ELISA applications. It has been validated for use in such assays and provides reliable detection of CD20.

5. What is the optimal dilution for using Rituximab in immunofluorescence?

   A: The optimal dilution for using Rituximab (TP-082CL) in immunofluorescence is typically 1:100 to 1:500. It is advisable to perform a dilution series to determine the best working concentration for your specific experimental conditions.

View the frequently asked questions answered by Creative Biolabs Support.

Citations

1. Chen, Haizhang, et al. "Detection and functional resolution of soluble immune complexes by an FcγR reporter cell panel." EMBO Molecular Medicine 14.1 (2022): e14182. https://doi.org/10.15252/emmm.202114182
   This study focuses on developing a robust and scalable test system to detect and quantify the bioactivity of soluble IgG immune complexes (sICs). These complexes play a significant role in autoimmune diseases such as systemic lupus erythematosus (SLE). The researchers created a comprehensive panel of reporter cell lines that enable the quantification of sIC reactivity through ELISA or flow cytometry. This system identified FcγRIIA(H) and FcγRIIIA as the most sensitive receptors to sICs, allowing for the detailed analysis of sIC size and stoichiometry. The assay has shown predictive capabilities regarding the severity of SLE by analyzing sera from patients and mouse models, providing a sensitive tool to evaluate sICs in various settings.
   Creative Biolabs contributed to this study by providing the pharmaceutical-grade humanized monoclonal IgG1 antibody rituximab (Cat# TP-082CL). Rituximab was crucial for generating soluble immune complexes (sICs) in the experiments. The study utilized recombinant CD20, obtained as a peptide containing the binding region of rituximab, to form these sICs. This setup allowed the researchers to create and analyze the immune complexes accurately. The high-quality rituximab provided by Creative Biolabs enabled the development of a sensitive and specific assay for detecting Fcγ receptor activation, which was essential for the study's success in evaluating the bioactivity and size of sICs.

Cite This Product

To accurately reference this product in your publication, please use the following citation information:

(Creative Biolabs Cat# TP-082CL, RRID: AB_3112049)

Submit Your Publication

Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.

Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

MSDS

COA

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

See other products for "IGHG1"

Select a product category from the dropdown menu below to view related products.