Anti-Human CD98 Recombinant Antibody (VB2-169) (CAT#: TAB-377LC)

This product is a recombinant monoclonal antibody targeting SLC3A2. The KD value was calculated to be 56 nM. antibody internalization was assessed by thermofluorescent labeling on flow cytometry.


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FC

Figure 1 shows the binding affinity of VB2-169. A-549 cells were incubated with various concentrations of (A) VB2-169 and the medium fluorescence was obtained by flow cytometry. The affinity of (B) VB2-169 was calculated using the Lineweaver- Burk method.

Figure 1 shows the binding affinity of VB2-169. A-549 cells were incubated with various concentrations of (A) VB2-169 and the medium fluorescence was obtained by flow cytometry. The affinity of (B) VB2-169 was calculated using the Lineweaver- Burk method.

FC

Figure 2 shows the biological activity of VB6-169 and VB6-170 as measured by flow cytometry and compared to VB6-845.

Figure 2 shows the biological activity of VB6-169 and VB6-170 as measured by flow cytometry and compared to VB6-845.

The reactivity and specificity of VB6-169 and VB6-170 was assessed with positive cell line SKOV-3 and negative cell line NCI-H460. A shift in median fluorescence with positive cell line, SKOV-3 was observed after incubation with VB6-169, VB6- 170 and VB6-845. In contrast, a marginal shift was observed with PBS as well as with the negative cell line, NCI-H460.

ELISA

Figure 3 shows the results of a competition assay.

Figure 3 shows the results of a competition assay.

Competition assay using VB6-169 and VB2-169, VB2-170 and 4B5 scFv with A-549 cells. A-549 cells were incubated with a fixed concentration of VB6-169 (10 μg/mL) and increasing concentrations of VB2-169 (black circle), VB2-170 (open circle), and 4B5 (black triangle) ranging from 0 to 100 μg/mL.


Specifications

  • Host Species
  • Human
  • Type
  • Human antibody
  • Specificity
  • Human
  • Clone
  • VB2-169
  • Applications
  • ELISA, FC
  • Related Disease
  • Prostate Cancer

Applications

  • Application Notes
  • This antibody has been reported for use in Enzyme-linked Immunosorbent Assay, Flow Cytometry.

Target

  • Alternative Names
  • 4F2; CD98; MDU1; 4F2HC; 4T2HC; NACAE; CD98HC; SLC3A2

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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See other products for "Clone VB2-169"

See other products for "CD98"

Chimeric Antibody

Recombinant Antibody

CAT Product Name Application Type
HPAB-0617-YJ Human Anti-CD98 Recombinant Antibody (clone CP3-10) ELISA, IF, IP, Inhib, FuncS Mouse IgG
HPAB-0618-YJ Human Anti-CD98 Recombinant Antibody (clone chCP3-10) ELISA, IF, IP, Inhib, FuncS Chimeric (mouse/human) IgG1
FAMAB-1568CQ Human Anti-CD98 Recombinant Antibody (clone CP1-5) ELISA Human IgG1
FAMAB-1571CQ Mouse Anti-CD98 Recombinant Antibody (clone RL388) IP, Stim, FC Mouse IgG2a

Fab Fragment Antibody

CAT Product Name Application Type
HPAB-0617-YJ-F(E) Human Anti-CD98 Recombinant Antibody (clone CP3-10); Fab Fragment ELISA, IF, IP, Inhib, FuncS Human Fab
HPAB-0618-YJ-F(E) Human Anti-CD98 Recombinant Antibody (clone chCP3-10); Fab Fragment ELISA, IF, IP, Inhib, FuncS Chimeric (mouse/human) Fab

scFv Fragment Antibody

CAT Product Name Application Type
HPAB-0617-YJ-S(P) Human Anti-CD98 Recombinant Antibody (clone CP3-10); scFv Fragment ELISA, IF, IP, Inhib, FuncS Human scFv
HPAB-0618-YJ-S(P) Mouse Anti-CD98 Recombinant Antibody (clone chCP3-10); scFv Fragment ELISA, IF, IP, Inhib, FuncS Mouse scFv

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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