Anti-Human CD98 Recombinant Antibody (VB2-169) (CAT#: TAB-377LC)

Figure 1 shows the binding affinity of VB2-169. A-549 cells were incubated with various concentrations of (A) VB2-169 and the medium fluorescence was obtained by flow cytometry. The affinity of (B) VB2-169 was calculated using the Lineweaver- Burk method.

Figure 2 shows the biological activity of VB6-169 and VB6-170 as measured by flow cytometry and compared to VB6-845.

The reactivity and specificity of VB6-169 and VB6-170 was assessed with positive cell line SKOV-3 and negative cell line NCI-H460. A shift in median fluorescence with positive cell line, SKOV-3 was observed after incubation with VB6-169, VB6- 170 and VB6-845. In contrast, a marginal shift was observed with PBS as well as with the negative cell line, NCI-H460.

Figure 3 shows the results of a competition assay.

Competition assay using VB6-169 and VB2-169, VB2-170 and 4B5 scFv with A-549 cells. A-549 cells were incubated with a fixed concentration of VB6-169 (10 μg/mL) and increasing concentrations of VB2-169 (black circle), VB2-170 (open circle), and 4B5 (black triangle) ranging from 0 to 100 μg/mL.