Anti-Human CLEC2D Recombinant Antibody (CAT#: TAB-173CL)

Recombinant Chimeric (human/mouse) monoclonal antibody expressed in CHO binding to Human CLEC2D. This is a monoclonal antibody that is capable of specifically binding to lectin-like transcript 1 (LLT1), to polynucleotides encoding such antibodiess and to cells that express such antibodiess. antibodiess have utility in the treatment of autoimmune diseases and cancer, in which LLT1- and CD161-expressing cells play a role in disease pathogenesis.


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DB

Figure 1 Anti-Human CLEC2D Recombinant Antibody (TAB-173CL-Fc silenced) in DB

Figure 1 Anti-Human CLEC2D Recombinant Antibody (TAB-173CL-Fc silenced) in DB

Dot Blot analysis of TAB-173CL-Fc silenced was performed by coating with human CLEC2D protein (CLD-H5253, acrobiosystems).
TAB-173CL-Fc silenced incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-Human IgG as a secondary antibody (1: 2000)

WB

Figure 2 Anti-Human CLEC2D Recombinant Antibody (TAB-173CL-Fc silenced) in WB

Figure 2 Anti-Human CLEC2D Recombinant Antibody (TAB-173CL-Fc silenced) in WB

Western blot analysis of TAB-173CL-Fc silenced was performed by loading human CLEC2D protein (CLD-H5253, acrobiosystems) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with TAB-173CL-Fc silenced and HRP goat anti-Human IgG as a secondary antibody (1: 2000). Chemiluminescent detection was performed.

Lane 1: Reducing antigen (0.1 μg)
Lane 2: Reducing antigen (0.3 μg)
Lane 3: Reducing antigen (0.6 μg)

ELISA

Figure 3 Anti-Human CLEC2D Recombinant Antibody (TAB-173CL-Fc silenced) in ELISA

Figure 3 Anti-Human CLEC2D Recombinant Antibody (TAB-173CL-Fc silenced) in ELISA

ELISA analysis of TAB-173CL-Fc silenced was performed by coating with human CLEC2D protein (CLD-H5253, acrobiosystems). Then blocked with BSA and incubated with TAB-173CL-Fc silenced. The HRP-conjugated goat anti-Human IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.

FC

Figure 4 Anti-Human CLEC2D Recombinant Antibody (TAB-173CL-Fc silenced) in FC

Figure 4 Anti-Human CLEC2D Recombinant Antibody (TAB-173CL-Fc silenced) in FC

Flow cytometry analysis of Daudi cells with purified antibody TAB-173CL-Fc silenced (TE221587-1, Blue). Cells were incubated with TAB-173CL-Fc silenced (5 μg/5x10^5 cells) for 30 min at 4°C. The secondary antibody used was FITC-Goat Anti-Human IgG Fc antibody (ab97224, abcam) for 30 min at 4°C.


Specifications

  • Host Species
  • mouse
  • Derivation
  • Chimeric (human/mouse)
  • Type
  • Antibody
  • Species Reactivity
  • Human
  • Related Disease
  • Autoimmune Diseases

Product Property

  • Purity
  • 90–95% (SDS-PAGE)
  • Storage
  • ≤1 year at -20°C.

Target

  • Alternative Names
  • CLEC2D; C-type lectin domain family 2, member D; CLAX; LLT1; OCIL; C-type lectin domain family 2 member D; LLT-1; C-type lectin related f; lectin-like transcript 1; lectin-like NK cell receptor; osteoclast inhibitory lectin; C-type lectin superfamily 2, m

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-0720 Hi-Affi™ Rabbit Anti-CLEC2D Recombinant Antibody (clone DS720AB) WB, FC Rabbit IgG

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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