Anti-Human CLEC2D Recombinant Antibody
CAT#: TAB-173CL
Recombinant Chimeric (human/mouse) monoclonal antibody expressed in CHO binding to Human CLEC2D. This is a monoclonal antibody that is capable of specifically binding to lectin-like transcript 1 (LLT1), to polynucleotides encoding such antibodiess and to cells that express such antibodiess. antibodiess have utility in the treatment of autoimmune diseases and cancer, in which LLT1- and CD161-expressing cells play a role in disease pathogenesis.
Tested Data
Gene Expression
DB
Figure 1 Anti-Human CLEC2D Recombinant Antibody (TAB-173CL-Fc silenced) in DB
Dot Blot analysis of TAB-173CL-Fc silenced was performed by coating with human CLEC2D protein (CLD-H5253, acrobiosystems).
TAB-173CL-Fc silenced incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-Human IgG as a secondary antibody (1: 2000)
WB
Figure 2 Anti-Human CLEC2D Recombinant Antibody (TAB-173CL-Fc silenced) in WB
Western blot analysis of TAB-173CL-Fc silenced was performed by loading human CLEC2D protein (CLD-H5253, acrobiosystems) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with TAB-173CL-Fc silenced and HRP goat anti-Human IgG as a secondary antibody (1: 2000). Chemiluminescent detection was performed.
Lane 1: Reducing antigen (0.1 μg)
Lane 2: Reducing antigen (0.3 μg)
Lane 3: Reducing antigen (0.6 μg)
ELISA
Figure 3 Anti-Human CLEC2D Recombinant Antibody (TAB-173CL-Fc silenced) in ELISA
ELISA analysis of TAB-173CL-Fc silenced was performed by coating with human CLEC2D protein (CLD-H5253, acrobiosystems). Then blocked with BSA and incubated with TAB-173CL-Fc silenced. The HRP-conjugated goat anti-Human IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
FC
Figure 4 Anti-Human CLEC2D Recombinant Antibody (TAB-173CL-Fc silenced) in FC
Flow cytometry analysis of Daudi cells with purified antibody TAB-173CL-Fc silenced (TE221587-1, Blue). Cells were incubated with TAB-173CL-Fc silenced (5 μg/5x10^5 cells) for 30 min at 4°C. The secondary antibody used was FITC-Goat Anti-Human IgG Fc antibody (ab97224, abcam) for 30 min at 4°C.
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Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/47584/747_H3_2_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human colon
Immunohistochemical staining of human colon shows strong positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/17649/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/47584/714_H3_1_blue_red_green.jpg
Subcellular Location and Protein Expression
Figure 4 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/47584/713_H3_2_blue_red_green.jpg
Normal Tissue
Figure 5 Colon
Glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Peripheral nerve/ganglion
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/17649/40429_A_9_3.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/17649/40429_A_8_5.jpg
Normal Tissue
Figure 7 Testis
Cells in seminiferous ducts
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/17649/40429_A_4_6.jpg
Normal Tissue
Figure 8 Lymph node
Germinal center cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/17649/40429_A_7_8.jpg
RNA Expression
Figure 9 RNA cell line category: Group enriched (Daudi, HDLM-2, Karpas-707, U-698)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000069493-CLEC2D
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Specifications
- Host Species
- mouse
- Derivation
- Chimeric (human/mouse)
- Type
- Antibody
- Species Reactivity
- Human
- Related Disease
- Autoimmune Diseases
Product Property
- Purity
- 90–95% (SDS-PAGE)
- Storage
- ≤1 year at -20°C.
Target
- Alternative Names
- CLEC2D; C-type lectin domain family 2, member D; CLAX; LLT1; OCIL; C-type lectin domain family 2 member D; LLT-1; C-type lectin related f; lectin-like transcript 1; lectin-like NK cell receptor; osteoclast inhibitory lectin; C-type lectin superfamily 2, m
- Gene ID
- 29121
- UniProt ID
- Q9UHP7
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Da**d11.Sep,24
Suitable for FACS
The Daudi cells were incubated with the antibody (TAB-173CL, 10 μg/1x10⁶ cells) for 30 min at 4°C.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
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| TAB-0684CL | Mouse Anti-CLEC2D Recombinant Antibody (TAB-0684CL) | FC, ELISA | Mouse IgG1 |
| TAB-0684CL-S(P) | Mouse Anti-CLEC2D Recombinant Antibody; scFv Fragment (TAB-0684CL-S(P)) | FC, ELISA | Mouse scFv |
| TAB-0685CL-S(P) | Mouse Anti-CLEC2D Recombinant Antibody; scFv Fragment (TAB-0685CL-S(P)) | FC, ELISA | Mouse scFv |
| TAB-0684CL-F(E) | Mouse Anti-CLEC2D Recombinant Antibody; Fab Fragment (TAB-0684CL-F(E)) | FC, ELISA | Mouse Fab |
| TAB-0685CL-F(E) | Mouse Anti-CLEC2D Recombinant Antibody; Fab Fragment (TAB-0685CL-F(E)) | FC, ELISA | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1109MZ | Recombinant Mouse Anti-Mouse CLEC2D Antibody (clone 166C1309) | WB | Mouse antibody |
| HPAB-0016-FY | Mouse Anti-CLEC2D Recombinant Antibody (HPAB-0016-FY) | FC | Mouse IgG1 |
| HPAB-650-FY | Mouse Anti-CLEC2D Recombinant Antibody (HPAB-650-FY) | WB | Mouse IgG |
| VS-0723-WK141 | Human Anti-CLEC2D Recombinant Antibody (VS-0723-WK141) | FC | Chimeric (rabbit/human) IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0720 | Hi-Affi™ Rabbit Anti-CLEC2D Recombinant Antibody (clone DS720AB) | WB, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0016-FY-S(P) | Mouse Anti-CLEC2D Recombinant Antibody; scFv Fragment (HPAB-0016-FY-S(P)) | FC | Mouse scFv |
| HPAB-650-FY-S(P) | Mouse Anti-CLEC2D Recombinant Antibody; scFv Fragment (HPAB-650-FY-S(P)) | WB | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0016-FY-F(E) | Mouse Anti-CLEC2D Recombinant Antibody; Fab Fragment (HPAB-0016-FY-F(E)) | FC | Mouse Fab |
| HPAB-650-FY-F(E) | Mouse Anti-CLEC2D Recombinant Antibody; Fab Fragment (HPAB-650-FY-F(E)) | WB | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC310 | Recombinant Anti-CLEC2D Vesicular Antibody, EV Displayed (VS-0425-YC310) | ELISA, FC, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1495 | Anti-CLEC2D Immunohistochemistry Kit | IHC |
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