Anti-Human CLEC2D Recombinant Antibody (CAT#: TAB-173CL)
Recombinant Chimeric (human/mouse) monoclonal antibody expressed in CHO binding to Human CLEC2D. This is a monoclonal antibody that is capable of specifically binding to lectin-like transcript 1 (LLT1), to polynucleotides encoding such antibodiess and to cells that express such antibodiess. antibodiess have utility in the treatment of autoimmune diseases and cancer, in which LLT1- and CD161-expressing cells play a role in disease pathogenesis.
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DB
Figure 1 Anti-Human CLEC2D Recombinant Antibody (TAB-173CL-Fc silenced) in DB
Dot Blot analysis of TAB-173CL-Fc silenced was performed by coating with human CLEC2D protein (CLD-H5253, acrobiosystems).
TAB-173CL-Fc silenced incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-Human IgG as a secondary antibody (1: 2000)
WB
Figure 2 Anti-Human CLEC2D Recombinant Antibody (TAB-173CL-Fc silenced) in WB
Western blot analysis of TAB-173CL-Fc silenced was performed by loading human CLEC2D protein (CLD-H5253, acrobiosystems) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with TAB-173CL-Fc silenced and HRP goat anti-Human IgG as a secondary antibody (1: 2000). Chemiluminescent detection was performed.
Lane 1: Reducing antigen (0.1 μg)
Lane 2: Reducing antigen (0.3 μg)
Lane 3: Reducing antigen (0.6 μg)
ELISA
Figure 3 Anti-Human CLEC2D Recombinant Antibody (TAB-173CL-Fc silenced) in ELISA
ELISA analysis of TAB-173CL-Fc silenced was performed by coating with human CLEC2D protein (CLD-H5253, acrobiosystems). Then blocked with BSA and incubated with TAB-173CL-Fc silenced. The HRP-conjugated goat anti-Human IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
FC
Figure 4 Anti-Human CLEC2D Recombinant Antibody (TAB-173CL-Fc silenced) in FC
Flow cytometry analysis of Daudi cells with purified antibody TAB-173CL-Fc silenced (TE221587-1, Blue). Cells were incubated with TAB-173CL-Fc silenced (5 μg/5x10^5 cells) for 30 min at 4°C. The secondary antibody used was FITC-Goat Anti-Human IgG Fc antibody (ab97224, abcam) for 30 min at 4°C.
Specifications
- Host Species
- mouse
- Derivation
- Chimeric (human/mouse)
- Type
- Antibody
- Species Reactivity
- Human
- Related Disease
- Autoimmune Diseases
Product Property
- Purity
- 90–95% (SDS-PAGE)
- Storage
- ≤1 year at -20°C.
Target
- Alternative Names
- CLEC2D; C-type lectin domain family 2, member D; CLAX; LLT1; OCIL; C-type lectin domain family 2 member D; LLT-1; C-type lectin related f; lectin-like transcript 1; lectin-like NK cell receptor; osteoclast inhibitory lectin; C-type lectin superfamily 2, m
- Gene ID
- 29121
- UniProt ID
- Q9UHP7
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Mouse Antibody Products
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Fab Antibody Products
Extracellular Vesicle (EV) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0684CL | Mouse Anti-CLEC2D Recombinant Antibody (TAB-0684CL) | FC, ELISA | Mouse IgG1 |
| TAB-0685CL | Mouse Anti-CLEC2D Recombinant Antibody (TAB-0685CL) | FC, ELISA | Mouse IgG1 |
| TAB-0684CL-S(P) | Mouse Anti-CLEC2D Recombinant Antibody; scFv Fragment (TAB-0684CL-S(P)) | FC, ELISA | Mouse scFv |
| TAB-0685CL-S(P) | Mouse Anti-CLEC2D Recombinant Antibody; scFv Fragment (TAB-0685CL-S(P)) | FC, ELISA | Mouse scFv |
| TAB-0684CL-F(E) | Mouse Anti-CLEC2D Recombinant Antibody; Fab Fragment (TAB-0684CL-F(E)) | FC, ELISA | Mouse Fab |
Customer Reviews and Q&As
Customer Review
Da**d
Suitable for FACS
Suitable for FACS
The Daudi cells were incubated with the antibody (TAB-173CL, 10 μg/1x10⁶ cells) for 30 min at 4°C.
11.Sep,24
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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