Anti-DENV2 E Recombinant Antibody (MRO-1132LC) (CAT#: MRO-1132LC)

Figure 1 Virus neutralization and mode of action of 9F12.

Pre- and post-adsorption assay. Pre-incubated mixtures of DENV-2 with mAb 9F12 (open squares) or 4G2 (open circles) were dispensed onto Vero cell monolayers at 4 °C for 1 h in the pre-adsorption assay. The virus antibody mixture was replaced with diluted virus in the post-adsorption assay, followed by washing and addition of either mAb 9F12 (filled squares) or 4G2 (filled circles).

On a mouse monoclonal antibody that neutralizes all four dengue virus serotypes

Figure 2 Binding of DENV-1–4 and WNV to 9F12.

The assay was carried out in triplicate and the values displayed represent the average. Error bars, sd.

On a mouse monoclonal antibody that neutralizes all four dengue virus serotypes

Figure 3 Epitope mapping.

Flow cytometry histograms of mAbs binding to yeast expressing wild-type or mutant domain III. The following antibodies were used as negative and positive staining controls, respectively, for each of the indicated yeast based on data from a prior publication: wild-type, WNV E16 and 3H5-1; K305E, 1A1D-2 and 3H5-1; K307C, 1A1D-2 and 3H5-1; K310E, 1A1D-2 and 3H5-1; P384A, 3H5-1 and 5A2-7; G330D, 6B6-10 and 3H5-1. In each case, staining with 9F12 is shown in red. The data are representative of three independent experiments.

On a mouse monoclonal antibody that neutralizes all four dengue virus serotypes

Figure 4 ELISA results of the antibody.

Elisa for C9 IgG (30 nmol/L) against DENV2 whole virus particles competed with increasing concentrations of mouse 9F12 and 3H5 IgG. C9 IgG binding was detected with an anti-human horseradish peroxidase (HRP) conjugate.

Phage Display Approaches for the Isolation of Monoclonal Antibodies Against Dengue Virus Envelope Domain III from Human and Mouse Derived Libraries