Hi-Affi™ Rabbit Anti-DLAT Recombinant Antibody (clone DS993AB)

Name: Hi-Affi™ Rabbit Anti-DLAT Recombinant Antibody (clone DS993AB)
SKU: MOR-0993
Rating: 5 (1 reviews)

CAT#: MOR-0993

Recombinant Rabbit anti-DLAT monoclonal antibody for WB, FC, ICC/IF, IP, excellent for producing superior results. Hi-Affi™ antibodies are developed from different sources, such as B cells of immunized animals, hybridoma, phage display library. The application of recombinant technology avoids the traditional ascitic fluid approach, which achieves the antibody generation in a humane way with much larger scale, greater specificity and sensitivity, and not susceptible to cell-line drift or lot-to-lot variation.

Gene Expression

Subcellular Location Figure 1 IF staining of human cell line U-251 MG

Immunofluorescent staining of human cell line U-251 MG shows localization to mitochondria.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/40786/754_D6_3_selected.jpg

Subcellular Location Figure 2 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/62766/1191_F12_2_selected.jpg

Subcellular Location Figure 3 IHC staining of human stomach

Immunohistochemical staining of human stomach, lower shows strong granular cytoplasmic positivity in glandular cells.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/40786/ihc_selected.jpg

Subcellular Location Figure 4 IF staining of human cell line A-431

Immunofluorescent staining of human cell line A-431 shows localization to mitochondria.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3782/671_G12_1_blue_red_green.jpg

Normal Tissue Figure 5 Cerebral cortex

Neuronal cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3782/11061_B_8_5.jpg

Normal Tissue Figure 6 Oral mucosa

Squamous epithelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/40786/125165_A_7_1.jpg

Normal Tissue Figure 7 Colon

Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/40786/125165_A_9_3.jpg

Normal Tissue Figure 8 Liver

Cholangiocytes
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/40786/125165_A_9_4.jpg

Normal Tissue Figure 9 Testis

Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/40786/125165_A_9_5.jpg

Normal Tissue Figure 10 Lymph node

Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3782/11061_A_9_8.jpg

RNA Expression Figure 11 RNA cell line category: Low cell line specificity

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000150768-DLAT

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Specifications

Host Species
Rabbit

Derivation
Phage display library

Type
Rabbit IgG

Specificity
Human DLAT

Species Reactivity
Mouse, Rat, Human

Clone
DS993AB

Applications
WB, FC, ICC, IF, IP

Conjugate
Unconjugated

Product Property

Purification
Purified with Absolute Mag™ Protein A Agarose

Purity
>95% as determined by SDS-PAGE

Concentration
Please refer to the vial label for the specific concentration.

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Alternative Names
Dihydrolipoamide S-Acetyltransferase; Dihydrolipoamide Acetyltransferase Component Of Pyruvate Dehydrogenase Complex; 70 KDa Mitochondrial Autoantigen Of Primary Biliary Cirrhosis; E2 Component Of Pyruvate Dehydrogenase Complex; Pyruvate Dehydrogenase Complex Component E2; M2 Antigen Complex 70 KDa Subunit; EC 2.3.1.12; PDC-E2; PDCE2; DLTA; PBC; Dihydrolipoyllysine-Residue Acetyltransferase Component Of Pyruvate Dehydrogenase Complex Mitochondrial; EC 2.3.1

Gene ID
1737

UniProt ID
P10515

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Downloadable Resources

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - MOR-0993.pdf

MSDS

MSDS - MOR-0993.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

Protocol & Troubleshooting

Immunofluorescence (IF)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

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CAT	Product Name	Application	Type
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