Mouse Anti-DNP Recombinant Antibody (clone SPE7)
CAT#: PABZ-033
Recombinant Mouse Antibody (SPE7) is capable of binding to DNP (2,4-dinitrophenyl), expressed in Chinese Hamster Ovary cells (CHO). A monoclonal immunoglobulin E (IgE) raised against a 2,4-dinitrophenyl (DNP) hapten. SPE7 exhibits high affinity [a dissociation constant (Kd) of 20 nM] and specificity toward DNP; its affinity for close analogs of DNP, such as 2-nitrophenol (NP) and 2-nitro-4-iodophenol, is negligible(Kd>100 M).


Specifications
- Immunogen
- 2,4-Dinitrophenol
- Host Species
- Mouse
- Type
- Mouse IgE
- Clone
- SPE7
- Applications
- ELISA
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody SPE7 has been reported in applications of Flow Cytometry, Enzyme-linked Immunosorbent Assay. It's recommended that the optimal antibody concentration, dilution, incubition time etc. are best to be carefully titrated in specific assays.
Flow Cytometry: It's reported that this antibody has been tested in flow cytometry for detecting MAPK and BMCMCs. The concentration of SPE7 was 5 μg/ml.
Enzyme-linked Immunosorbent Assay: The reported concentration of SPE7 was 5 μg/ml.
Target
- Alternative Names
- DNP; 2,4-Dinitrophenol; 2,4-DNP
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To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# PABZ-033, RRID: AB_3111707)
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
See other products for "Clone SPE7"
- CAT
- Product Name
See other products for "DNP"
Select a product category from the dropdown menu below to view related products.
CAT | Product Name | Application | Type |
---|---|---|---|
MOB-286 | Recombinant Anti-DNP Antibody | ELISA, IP, FuncS | IgG |
MHH-286 | Recombinant Human Anti-DNP Antibody | WB, FC, FuncS | IgG |
FAMAB-0091YC | Recombinant Mouse Anti-DNP Antibody (MOPC315) | WB | Mouse IgA |
FAMAB-0103CQ | Mouse Anti-DNP Recombinant Antibody (clone MOPC315) | WB | Mouse IgA |
VS-0224-XY37 | Rabbit Anti-DNP Recombinant Antibody (VS-0224-XY37) | WB, IF | Rabbit IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
MOB-286-F(E) | Recombinant Anti-DNP Antibody Fab Fragment | WB, ELISA, FuncS | Fab |
MHH-286-F(E) | Recombinant Human Anti-DNP Antibody Fab Fragment | FC, ELISA, FuncS | Fab |
PFBZ-033 | Mouse Anti-DNP Recombinant Antibody (clone SPE7); Fab Fragment | ELISA | Mouse Fab |
FAMAB-0091-YC-F(E) | Recombinant Mouse Anti-DNP Antibody Fab Fragment (MOPC315) | WB | Mouse Fab |
FAMAB-0103CQ-F(E) | Mouse Anti-DNP Recombinant Antibody (clone MOPC315); Fab Fragment | WB | Mouse Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
MOB-286-S(P) | Recombinant Anti-DNP Antibody scFv Fragment | FC, RIA, Biosensors, FuncS | scFv |
MHH-286-S(P) | Recombinant Human Anti-DNP Antibody scFv Fragment | ELISA, WB, IF, FuncS | scFv |
PSBZ-033 | Mouse Anti-DNP Recombinant Antibody (clone SPE7); scFv Fragment | ELISA | Mouse scFv |
FAMAB-0091-YC-S(P) | Recombinant Mouse Anti-DNP Antibody scFv Fragment (MOPC315) | WB | Mouse scFv |
FAMAB-0103CQ-S(P) | Mouse Anti-DNP Recombinant Antibody (clone MOPC315); scFv Fragment | WB | Mouse scFv |
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