Mouse Anti-DNP Recombinant Antibody (clone SPE7) (CAT#: PABZ-033)
Recombinant Mouse Antibody (SPE7) is capable of binding to DNP (2,4-dinitrophenyl), expressed in Chinese Hamster Ovary cells (CHO). A monoclonal immunoglobulin E (IgE) raised against a 2,4-dinitrophenyl (DNP) hapten. SPE7 exhibits high affinity [a dissociation constant (Kd) of 20 nM] and specificity toward DNP; its affinity for close analogs of DNP, such as 2-nitrophenol (NP) and 2-nitro-4-iodophenol, is negligible(Kd>100 M).
Specific Inquiry
Figure 1 Flow cytometry analysis
Cell surface expression of members of the IL-1R family on mouse mast cells. Naive BMCMCs were incubated with various concentration of H1-ε-26 IgE or SPE-7 IgE for 16 h. Cell surface expression of members of the IL-1R family on mast cells (i.e., c-kit⁺, FcεRα⁺ cells) was analyzed by flow cytometry. (A) Shaded areas, Isotype-matched control IgG mAb; bold lines, specific mAb. (B) Data are representative of similar results, which were obtained in the three experiments we performed, each with a different batch of BMCMCs (A), or show the average ± SD (n=3 different batches of BMCMCs) of values from the three experiments. *, P< 0.05, versus corresponding values for no IgE (0 g/ml) by Student's t-test; † , P < 0.05, versus corresponding values for control IgG staining by ANOVA. MFI, Mean fluorescence intensity.
Ho, L. H., Ohno, T., Oboki, K., Kajiwara, N., Suto, H., Iikura, M., ... & Nakae, S. (2007). IL‐33 induces IL‐13 production by mouse mast cells independently of IgE‐FcεRI signals. Journal of leukocyte biology, 82(6), 1481-1490.
Figure 2 IL-33 promotes mouse mast cell cytokine production with or without IgE.
Naive BMCMCs were cultured in the presence or absence of 10 μg/ml H1-ε-26 IgE or 5 μg/ml SPE-7 IgE plus various concentrations of IL-1β, IL-18, or IL-33 for 6 h. IL-4, IL-6, and IL-13 levels in culture supernatants of BMCMCs were measured by ELISA. Data are the average ± SD (n=3 different batches of BMCMCs). *, P < 0.05, versus the corresponding values for BMCMCs incubated without cytokine (indicated as "0") by Student's t-test; †, P < 0.05, versus the corresponding values for BMCMCs incubated with IL-1β or IL-18; ‡, P < 0.05, versus the corresponding values for BMCMCs incubated with H1-ε-26 or without IgE by ANOVA, respectively.
Ho, L. H., Ohno, T., Oboki, K., Kajiwara, N., Suto, H., Iikura, M., ... & Nakae, S. (2007). IL‐33 induces IL‐13 production by mouse mast cells independently of IgE‐FcεRI signals. Journal of leukocyte biology, 82(6), 1481-1490.
Specifications
- Immunogen
- 2,4-Dinitrophenol
- Host Species
- Mouse
- Type
- Mouse IgE
- Clone
- SPE7
- Applications
- ELISA
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody SPE7 has been reported in applications of Flow Cytometry, Enzyme-linked Immunosorbent Assay. It's recommended that the optimal antibody concentration, dilution, incubition time etc. are best to be carefully titrated in specific assays.
Flow Cytometry: It's reported that this antibody has been tested in flow cytometry for detecting MAPK and BMCMCs. The concentration of SPE7 was 5 μg/ml.
Enzyme-linked Immunosorbent Assay: The reported concentration of SPE7 was 5 μg/ml.
Target
- Alternative Names
- DNP; 2,4-Dinitrophenol; 2,4-DNP
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-286 | Recombinant Anti-DNP Antibody | ELISA, IP, FuncS | IgG |
| MHH-286 | Recombinant Human Anti-DNP Antibody | WB, FC, FuncS | IgG |
| FAMAB-0091YC | Recombinant Mouse Anti-DNP Antibody (MOPC315) | WB | Mouse IgA |
| FAMAB-0103CQ | Mouse Anti-DNP Recombinant Antibody (clone MOPC315) | WB | Mouse IgA |
| VS-0224-XY37 | Rabbit Anti-DNP Recombinant Antibody (VS-0224-XY37) | WB, IF | Rabbit IgG |
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To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# PABZ-033, RRID: AB_3111707)
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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