Recombinant Anti-DNP Antibody Fab Fragment (CAT#: MOB-286-F(E))

Bracaglia, Sara, et al. "Programmable, multiplexed DNA circuits supporting clinically relevant, electrochemical antibody detection." Acs Sensors 6.6 (2021): 2442-2448. https://doi.org/10.1021/acssensors.1c00790

This research presents the development of a programmable DNA circuit that enables electrochemical detection of specific antibodies with high sensitivity and selectivity. The system utilizes synthetic antigen-conjugated nucleic acid strands designed to trigger a strand displacement reaction upon antibody recognition, releasing a redox reporter-modified strand that produces a measurable electrochemical signal. This innovative approach achieves nanomolar detection limits, demonstrates specificity by generating signals only in the presence of target antibodies, and maintains functionality even in complex media such as 90% serum. The researchers successfully demonstrated the platform's versatility by detecting five different antibodies, including three clinically relevant ones, and showed its multiplexing capability using different redox reporters to simultaneously detect multiple antibodies without cross-talk.
Creative Biolabs provided anti-DNP Fab fragments that were used to validate the specificity of the DNA circuit system. These antibody fragments played an important role in the control experiments that demonstrated the mechanism relies on bivalent antibody binding, as the single-binding-site Fab fragments produced signals indistinguishable from antibody-free samples. This contribution helped establish the fundamental principle of the antibody-induced co-localization mechanism that drives the sensor's functionality, confirming that both antigen-binding sites of the antibody are required for proper detection, which is essential for the specificity and reliability of this electrochemical antibody detection platform.

Ranallo, Simona, Daniela Sorrentino, and Francesco Ricci. "Orthogonal regulation of DNA nanostructure self-assembly and disassembly using antibodies." Nature Communications 10.1 (2019): 5509. https://doi.org/10.1038/s41467-019-13104-6

This research demonstrates a rational strategy for controlling DNA nanostructure assembly and disassembly using specific IgG antibodies as molecular inputs. The scientists developed a system where binding of an antibody to a pair of antigen-conjugated split DNA input-strands induces their co-localization, reconstituting them into a functional unit that can initiate toehold strand displacement reactions. This approach is highly specific, rapid, and adaptable to different antibodies by simply changing the recognition elements attached to the DNA strands. The researchers successfully employed this antibody-regulated DNA circuit to control the assembly and disassembly of DNA tubular structures in an orthogonal way, demonstrating that two distinct DNA nanostructures could be independently controlled by different IgG antibodies (anti-Dig and anti-DNP) in the same solution.
Creative Biolabs provided anti-DNP Fab fragments (CAT#: MOB-286-F(E)) that were crucial for validating the specificity of the antibody-controlled DNA circuit. These Fab fragments, containing only a single antigen binding site, helped confirm that the co-localization effect induced by intact antibodies with their dual binding sites was essential for the DNA circuit activation. This validation was important in demonstrating that the mechanism relied on antibody-induced proximity rather than simple binding, supporting the fundamental principle of the antibody-controlled DNA nanotechnology approach which could potentially lead to applications in diagnostics, controlled drug release, and in-vivo imaging.