Mouse Anti-DOTA Recombinant Antibody (clone 2D12.5); Fab Fragment
CAT#: PFBW-133
Recombinant Mouse Antibody (2D12. 5) Fab Fragment is specific to Y-DOTA (1,4,7,10-tetraazacyclododecane-N,N',N'',N'''-tetraacetic acid), expressed in Chinese Hamster Ovary cells (CHO).
Specifications
- Immunogen
- 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid
- Host Species
- Mouse
- Type
- Mouse Fab
- Clone
- 2D12.5
- Applications
- ELISA
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- DOTA; Y-DOTA; 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid
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Excellent Molecular Biology
This antibody has produced results in molecular biology investigations with great specificity that are easy to understand and interpret. It's a trustworthy research aid.
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My experimental results were validated by the trustworthy and consistent control results obtained with this antibody. Very comforting!
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Utilizing this antibody has made my study much more effective. Time and resources are saved because to the reliable and excellent results.
Q&As
-
How should this antibody be aliquoted to ensure long-term storage?
A: To prevent recurrent freeze-thaw cycles, aliquot the antibody in small volumes that are enough for one or two trials, and keep the aliquots at -20°C or lower.
-
What controls should I include in my experiment?
A: Provide a negative control (target protein absent) and a positive control (target protein known to be expressed). Include an isotype control as well if you're doing immune-based experiments.
-
Can this antibody detect the protein in various cell lines?
A: Yes, the antibody should be able to identify the protein of interest as long as it is expressed in those cell lines. There may be a need for validation with particular cell lines.
View the frequently asked questions answered by Creative Biolabs Support.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# PFBW-133, RRID: AB_3111719)
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "Clone 2D12.5"
- CAT
- Product Name
See other products for "DOTA"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABW-133 | Mouse Anti-DOTA Recombinant Antibody (clone 2D12.5) | ELISA | Mouse IgG |
| HPAB-1251WJ | Mouse Anti-DOTA Recombinant Antibody (HPAB-1251WJ) | ELISA, Inhib | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBW-133 | Mouse Anti-DOTA Recombinant Antibody (clone 2D12.5); scFv Fragment | ELISA | Mouse scFv |
| HPAB-1251WJ-S(P) | Mouse Anti-DOTA Recombinant Antibody; scFv Fragment (HPAB-1251WJ-S(P)) | ELISA | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1251WJ-F(E) | Mouse Anti-DOTA Recombinant Antibody; Fab Fragment (HPAB-1251WJ-F(E)) | ELISA | Mouse Fab |
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