Human Anti-DR5 Recombinant Antibody (clone Apomab)
CAT#: PABL-453
Recombinant Human Antibody (Apomab) is capable of binding to DR5, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-DR5 mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-DR5 proteins mAb and CL of human kappa light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition. This antibody induces tumor cell apoptosis through DR5 and investigated the structural features of its interaction with DR5.
Published Data
Tested Data
Figure 1 ELISA analysis of Apomab binding to DR5.
Apomab binding to purified human IgG1 Fc fusion proteins based on the human Apo2L/TRAIL receptors DR5, DR4, DcR1, DcR2 and OPG (Genentech Inc.). Binding was determined in ELISA format: each receptor–Fc fusion was coated onto microtiter wells and incubated with a serial dilution of Apomab for 2 h at room temperature. Bound Apomab was then detected using a horseradish peroxidase-conjugated goat F(ab′)2 anti-human IgG antibody.
Adams, C., Totpal, K., Lawrence, D., Marsters, S., Pitti, R., Yee, S., ... & Compaan, D. (2008). Structural and functional analysis of the interaction between the agonistic monoclonal antibody Apomab and the proapoptotic receptor DR5. Cell death and differentiation, 15(4), 751.
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DB
Figure 3 Anti-DR5 Antibody (PABL-453) in Dot Blot
Dot Blot analysis of PABL-453 was performed by coating with Recombinant Human DR5 Protein (his Tag).
PABL-453 incubation concentration: 1 ng/μL.
The secondary antibody: HRP-Anti-Human IgG (H+L)
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Specifications
- Immunogen
- Human tumor necrosis factor receptor superfamily, member 10b
- Host Species
- Human
- Type
- Human IgG
- Specificity
- Human DR5
- Species Reactivity
- Human
- Clone
- Apomab
- Applications
- FC, ELISA
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- TNFRSF10B; tumor necrosis factor receptor superfamily, member 10b; DR5; CD262; KILLER; TRICK2; TRICKB; ZTNFR9; TRAILR2; TRICK2A; TRICK2B; TRAIL-R2; KILLERDR5; tumor necrosis factor receptor superfamily member 10B; Fas-like protein; death receptor 5; cytotoxic TRAIL receptor-2; TNF receptor superfamily member 10b; apoptosis inducing receptor TRAIL-R2; apoptosis inducing protein TRICK2A2B; TNF-related apoptosis-inducing ligand receptor 2; death domain containing receptor for TRAILApo-2L; tumor necrosis factor receptor-like protein ZTNFR9; p53-regulated DNA damage-inducible cell death receptor(killer);
- Gene ID
- 8795
- UniProt ID
- O14763
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "Clone Apomab"
- CAT
- Product Name
See other products for "DR5"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0245MC | Human Anti-DR5 Recombinant Antibody (clone YC037) | ELISA, WB, IHC, FuncS | |
| HPAB-0621LY | Mouse Anti-DR5 Recombinant Antibody (HPAB-0621LY) | FC | Mouse IgG |
| HPAB-0622LY | Mouse Anti-DR5 Recombinant Antibody (HPAB-0622LY) | FC | Mouse IgG |
| HPAB-0728LY | Human Anti-DR5 Recombinant Antibody (clone mAb1) | ELISA | Human IgG |
| HPAB-0729LY | Human Anti-DR5 Recombinant Antibody (clone mAb2) | ELISA | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBL-450 | Human Anti-DR5 Recombinant Antibody (clone Apomab); Fab Fragment | FC, ELISA | Human Fab |
| HPAB-0621LY-F(E) | Mouse Anti-DR5 Recombinant Antibody; Fab Fragment (HPAB-0621LY-F(E)) | FC | Mouse Fab |
| HPAB-0622LY-F(E) | Mouse Anti-DR5 Recombinant Antibody; Fab Fragment (HPAB-0622LY-F(E)) | FC | Mouse Fab |
| HPAB-0728LY-F(E) | Human Anti-DR5 Recombinant Antibody (clone mAb1); Fab Fragment | ELISA | Human Fab |
| HPAB-0729LY-F(E) | Human Anti-DR5 Recombinant Antibody (clone mAb2); Fab Fragment | ELISA | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-450 | Human Anti-DR5 Recombinant Antibody (clone Apomab); scFv Fragment | FC, ELISA | Human scFv |
| HPAB-0621LY-S(P) | Mouse Anti-DR5 Recombinant Antibody; scFv Fragment (HPAB-0621LY-S(P)) | FC | Mouse scFv |
| HPAB-0622LY-S(P) | Mouse Anti-DR5 Recombinant Antibody; scFv Fragment (HPAB-0622LY-S(P)) | FC | Mouse scFv |
| HPAB-0728LY-S(P) | Human Anti-DR5 Recombinant Antibody (clone mAb1); scFv Fragment | ELISA | Human scFv |
| HPAB-0729LY-S(P) | Human Anti-DR5 Recombinant Antibody (clone mAb2); scFv Fragment | ELISA | Human scFv |
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