Human Anti-DR5 Recombinant Antibody (clone YC037) (CAT#: MOB-0245MC)

Figure 3 Anti-DR5 Antibody (MOB-0245MC) in Dot Blot

Dot Blot analysis of MOB-0245MC was performed by coating with Recombinant Human DR5 Protein (his Tag).

MOB-0245MC incubation concentration: 1 ng/μL.
The secondary antibody: HRP-Anti-Human IgG (H+L)

Figure 1 Anti-DR5 Antibody (MOB-0245MC) in ELISA

ELISA analysis of MOB-0245MC was performed by coating with Recombinant Human DR5 Protein. Then blocked with BSA and incubated with Anti-Human DR5 Antibody (MOB-0245MC). The HRP-conjugated goat anti-human IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.

Figure 2 Anti-DR5 Antibody (MOB-0245MC) in WB

Western blot analysis of MOB-0245MC was performed by loading Recombinant Human DR5 Protein onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with MOB-0245MC and HRP Goat Anti-Human IgG as a secondary antibody. Chemiluminescent detection was performed.