Recombinant Human Anti-Dopamine transporter Antibody (9D5)
CAT#: PABL-452
Recombinant Human Antibody (9D5) is capable of binding to Dopamine transporter, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-Dopamine transporter mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-Dopamine transporter proteins mAb and CL of human kappa light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition.
Specifications
- Immunogen
- D. melanogaster Dopamine transporter
- Host Species
- Human
- Derivation
- Human
- Type
- IgG
- Specificity
- Tested positive against native Dopamine transporter
- Species Reactivity
- D. melanogaster
- Clone
- 9D5
- Applications
- Can be useful in applications such as: Western Blot; Enzyme-linked Immunosorbent Assay; Functional Study
Product Property
- Purity
- >95% by SDS-PAGE and HPLC analysis
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Target
- Alternative Names
- dopamine transporter; dopamine active transporter; DAT; SLC6A3
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Effective Dat Western Blot
Clone 9D5 worked well in our Western Blot assays to detect the Dopamine transporter. This recombinant human antibody, expressed in HEK 293 cells, showed specific binding to the target. It has been useful for our studies on dopamine reuptake mechanisms and synaptic function.
Versatile Functional Antibody
We used this antibody in functional studies (FuncS) and ELISA. It is a disulfide-linked dimer that mimics the natural antibody structure well. Its ability to bind native DAT has made it a key reagent in our research on neurotransmitter transporters and their regulation in the brain.
Q&As
-
What is the structure of this recombinant antibody?
A: This Recombinant Human Antibody (clone 9D5) exists as a disulfide-linked dimer of a heavy chain (HC) and a light chain (LC). It contains the VH from an anti-Dopamine transporter mAb and the CH1-3 region of human IgG1.
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Does this antibody bind native DAT?
A: Yes, this antibody has tested positive against native Dopamine transporter (DAT). It is suitable for Western Blot, ELISA, and Functional Studies to investigate dopamine reuptake mechanisms.
View the frequently asked questions answered by Creative Biolabs Support.
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Related Diseases
Amphetamine Addiction
Alcoholism
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "Clone 9D5"
- CAT
- Product Name
See other products for "DAT"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABL-059 | Recombinant Mouse Anti-D. melanogaster DAT Antibody (PABL-059) | IF | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-059 | Recombinant Mouse Anti-D. melanogaster DAT Antibody scFv Fragment (PSBL-059) | WB, ELISA, FuncS | scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBL-059 | Recombinant Mouse Anti-D. melanogaster DAT Antibody Fab Fragment (PFBL-059) | WB, ELISA, FuncS | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABL-476 | Recombinant Mouse Anti-Fruit fly DAT Antibody (9D5) | WB, IF, FuncS | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBL-449 | Recombinant Human Anti-Dopamine transporter Antibody Fab Fragment (9D5) | WB, ELISA, FuncS | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBL-473 | Recombinant Mouse Anti-Fruit fly DAT Antibody Fab Fragment (9D5) | WB, IF, FuncS | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-449 | Recombinant Human Anti-Dopamine transporter Antibody scFv Fragment (9D5) | WB, ELISA, FuncS | scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-473 | Recombinant Mouse Anti-Fruit fly DAT Antibody scFv Fragment (9D5) | WB, IF, FuncS | scFv |
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