Hi-Affi™ Recombinant Rabbit Anti-EBOV Monoclonal Antibody (SI273DS)
CAT#: MOR-4196
Recombinant Rabbit anti-EBOV monoclonal antibody for WB, ELISA, IHC-P, ICC/IF, IF, FCM , excellent for producing superior results. Hi-Affi™ antibodies are developed from different sources, such as B cells of immunized animals, hybridoma, phage display library. The application of recombinant technology avoids the traditional ascitic fluid approach, which achieves the antibody generation in a humane way with much larger scale, greater specificity and sensitivity, and not susceptible to cell-line drift or lot-to-lot variation.
Specifications
- Immunogen
- Recombinant Sudan ebolavirus EBOV-G/Glycoprotein Protein
- Host Species
- Rabbit
- Type
- IgG
- Specificity
- Ebola virus EBOV (Subtype Sudan, strain Gulu) Glycoprotein/GP1 (mucin domain deleted)
- Species Reactivity
- Ebola
- Clone
- SI273DS
- Applications
- Used for immunoassay techniques such as: Western Blot; Enzyme-linked Immunosorbent Assay; Immunohistochemistry (Paraffin); Immunocytochemistry/Immunofluorescence; Immunofluorescence; Flow Cytometry
- Conjugate
- Unconjugated
Product Property
- Purification
- Purified with Absolute Mag™ Protein A Agarose
- Purity
- > 95% as determined by both RP-HPLC and SDS-PAGE analysis.
- Format
- Liquid
- Storage
- Store at -20°C for long-term storage. Store at 4°C for up to one month. Avoid freeze/thaw cycles.
Applications
- Application Notes
- ELISA: 0.1-0.2 μg/ml
Target
- Alternative Names
- Ebola Virus
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Superior Recombinant Rabbit Antibody
The Hi-Affi recombinant rabbit antibody against EBOV demonstrated superior sensitivity in our Western Blot and ELISA assays compared to traditional antibodies. We used it to detect the Sudan ebolavirus glycoprotein, and the signal was strong and specific, likely due to the lack of cell-line drift.
Versatile For Ebola Virus Research
This monoclonal antibody was extremely versatile, working well in IHC (paraffin), Immunofluorescence, and Flow Cytometry. It targets the GP1 subunit effectively. The fact that it is a recombinant product derived from B cells gives us confidence in its specificity for the mucin domain-deleted glycoprotein.
Q&As
-
What technology is used to develop this antibody?
A: This Hi-Affi™ antibody is developed using recombinant technology, which avoids the traditional ascitic fluid approach. It is derived from sources like B cells or phage display libraries to ensure high specificity, sensitivity, and humane generation.
-
What specific strain of Ebola does this antibody target?
A: This antibody targets the Ebola virus EBOV (Subtype Sudan, strain Gulu) Glycoprotein/GP1. Specifically, it recognizes the mucin domain-deleted version of the glycoprotein.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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